光裸星虫不同组织同工酶谱分析

采用聚丙烯酰胺梯度凝胶垂直平板电泳对湛江地区的光裸星虫(Sipunculus nudus)3种组织(肠组织、体腔液和体壁肌肉)12种同工酶(SOD、EST、MDH、LDH、ALP、AMY、ME、ACP、G6PDH、SDH、ADH、GPI)进行检测分析。结果表明:检测的12种同工酶中的7种酶(SOD、EST、MDH、LDH、ALP、AMY、ME)具有明显的组织特异性;GPI在组织中表达不稳定,未检测到其他4种同工酶(ACP、G6PDH、SDH、ADH)的活性;光裸星虫酶谱带数目少,酶谱构型单一。并说明了对其种群内遗传生化特性进行系统研究的必要性。     

管角螺不同组织同工酶的研究

采用聚丙烯酰胺垂直板梯度凝胶电泳技术,对管角螺7种组织(外套膜、肌肉、肝脏、心脏、生殖腺和缠卵腺)的5种同工酶(EST、SOD、ME、MDH、LDH)进行了分析,结果表明:5种同工酶存在不同程度的组织特异性,SOD、EST、LDH在肝脏组织中有较高活性,而MDH、ME在心脏组织中活性较高。     

似刺鳊鮈发育过程中同工酶变化及组织分布特征分析

运用高pH不连续聚丙烯酰胺凝胶(PAGE)电泳技术分析了似刺鳊鮈胚胎、仔鱼发育过程中5种同工酶变化,并对成鱼的9种组织同工酶分布特征进行了检测。结果发现:除醇脱氢酶(ADH)外,其他4种酶(EST、LDH、MDH、SOD)在发育过程中均被检出,且呈现明显的阶段性特征;在成鱼的4种同工酶也存在明显的组织特异性。4种酶共检测到9个基因位点。     

管角螺不同组织同工酶的研究

采用聚丙烯酰胺垂直板梯度凝胶电泳技术，对管角螺7种组织（外套膜、肌肉、肝脏、心脏、生殖腺和缠卵腺）的5种同工酶（EST、SOD、ME、MDH、LDH）进行了分析，结果表明：5种同工酶存在不同程度的组织特异性，SOD、EST、LDH在肝脏组织中有较高活性，而MDH、ME在心脏组织中活性较高。     

月鳢个体发育早期4种同工酶的初步研究

采用垂直板聚丙烯酰胺凝胶电泳技术,对月鳢早期(从未受精卵至卵黄吸尽期)8个发育阶段的LDH、MDH、ADH、EST同工酶进行研究。结果表明:月鳢的早期发育阶段ADH酶谱相对稳定,均有两条谱带;LDH、MDH和EST三种同工酶的酶谱在神经胚期前均无明显变化,此后随发育进程逐渐复杂。     

月鳢个体发育早期4种同工酶的初步研究

采用垂直板聚丙烯酰胺凝胶电泳技术，对月鳢早期(从未受精卵至卵黄吸尽期)8个发育阶段的LDH、MDH、ADH、EST同工酶进行研究。结果表明：月鳢的早期发育阶段ADH酶谱相对稳定，均有两条谱带；LDH、MDH和EST三种同工酶的酶谱在神经胚期前均无明显变化，此后随发育进程逐渐复杂。     

军曹鱼不同组织5种同工酶的研究

采用聚丙烯酰胺垂直凝胶电泳法对军曹鱼的几种组织 (脑、心肌、肾、肌肉、性腺、肝、血液、胰、脾 )的 5种同工酶 (LDH、MDH、ME、POD和EST)进行了分析 ,结果表明 :5种同工酶在军曹鱼中都具有明显的组织特异性 ,这些特异性与其组织特定生理功能相关。与其他硬骨鱼类相比 ,军曹鱼的酶谱简单得多 ,这可能与其种属多样性相关。     

军曹鱼不同组织5种同工酶的研究

采用聚丙烯酰胺垂直凝胶电泳法对军曹鱼的几种组织（脑，心肌，肾，肌肉，性腺，肝，血液，胰，脾）的5种同工酶（LDH，MDH，ME，POD和EST）进行了分析。结果表明：5种同工酶在军曹鱼中都具有明显的组织特异性，这些特异性与其组织特定生理功能相关，与其他硬骨鱼类相比，军曹鱼的酶谱简单得多，这可能与其种属多样性相关。     

企鹅珍珠贝同工酶表达多态性的初步研究

采用垂直板状聚丙烯酰胺梯度凝胶电泳,对企鹅珍珠贝鳃组织的酯酶(EST)、葡萄糖-6磷酸脱氢酶(G6PD)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)5种同工酶进行了检测,并对酶谱特征进行了生化遗传分析.酶谱表型显示企鹅珍珠贝的同工酶呈现多态现象,同工酶表达上的多态性表明企鹅珍珠贝具有较丰富的遗传多样性.     

企鹅珍珠贝同工酶表达多态性的初步研究

采用垂直板状聚丙烯酰胺梯度凝胶电泳，对企鹅珍珠贝鳃组织的酯酶(EST)、葡萄糖-6磷酸脱氢酶(G6PD)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)5种同工酶进行了检测，并对酶谱特征进行了生化遗传分析。酶谱表型显示企鹅珍珠贝的同工酶呈现多态现象，同工酶表达上的多态性表明企鹅珍珠贝具有较丰富的遗传多样性。     

Isozyme Analysis of Spotted Halibut Verasper variegatus Temminck et Schlegel

To investigate the tissue-specificities of isozymes and the genetic structure of wild spotted halibut ( Verasper variegatus) population, horizontal starch gel electrophoresis was performed on 45 individuals collected in part of the Yellow Sea. The performances of 17 isozymes in 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The results showed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes (AAT, ADH, EST, GPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues (eye, skeleton muscle, liver and heart) were selected for genetic analysis. Fourteen isozymes are encoded by 20 loci, and 9 of them are poly-morphic. The polymorphic loci are AAT-1*, GPI-2*, G3PDH*, IDHP-1*, LDH*, MPI*, PGM-1*, PGM-2* and SDH*, and the proportion of polymorphic loci is 0.450 0 (P0.99). The mean values of observed and expected heterozygosities are 0.027 8 and 0.026 5, respectively and the average effective number of alleles is 1.067 5.     

Isozyme analysis of spotted halibut Verasper variegatus temminck et schlegel

To investigate the tissue-specificities of isozymes and the genetic structure of wild spotted halibut (Verasper variegates) population, horizontal starch gel electrophoresis was performed on 45 individuals collected in part of the Yellow Sea. The performances of 17 isozymes in 8 kinds of tissues or organs were screened preliminarily in a TC-7.0 buffer system. The results showed that the screened isozymes displayed remarkable tissue-specificities. Finally, 14 enzymes (AAT, ADH, EST, GPI, G3PDH, IDHP, LAP, LDH, MDH, MPI, PGDH, PGM, SDH and SOD) and 4 kinds of tissues (eye, skeleton muscle, liver and heart) were selected for genetic analysis. Fourteen isozymes are encoded by 20 loci, and 9 of them are polymorphic. The polymorphic loci are AAT-1*. GPI-2* G3PDH*, IDHP-1*, LDH*, MPI*, PGM-1*. PGM-2* and SDH*, and the proportion of polymorphic loci is 0.4500 (P0.99). The mean values of observed and expected heterozygosities are 0.027 8 and 0.026 5, respectively and the average effective number of alleles is 1.067 5.     

GENETIC DIFFERENTIATION WITHIN ERIOCHOIR SINENSIS (MILNE,EDWARDS) REVEALED BY ALLOZYME AND RAPD

ＩＮＴＲＯＤＵＣＴＩＯＮＥｒｉｏｃｈｏｉｒｓｉｎｅｎｓｉｓｉｎＣｈｉｎａｉｓｃｏｍｍｏｎｌｙｃａｌｌｅｄｍｉｔｔｅｎｃｒａｂ .Ｉｔｉｓａｎａｄｒｏｍｏｕｓ,ｗｉｔｈｉｎｄｉｖｉｄｕａｌｓｍｏｖｉｎｇｆｒｏｍｆｒｅｓｈｔｏｍａｒｉｎｅｗａｔｅｒｔｏｓｐａｗｎ .Ｔｈｅｒｅａｒｅｔｗｏｃｏｍｍｅｒｃｉａｌｐｏｐｕｌａｔｉｏｎｓ:ＣｈａｎｇｊｉａｎｇＲｉｖ ｅｒ (ＣＲ)ａｎｄＬｉａｏｈｅＲｉｖｅｒ (ＬＲ) .Ｔｈｅｓｅｔｗｏｐｏｐｕｌａｔｉｏｎｓａｒｅｖｅｒｙｄｉｆｆｉｃｕｌｔｔｏｉｄｅｎｔｉｆｙｆｒｏｍｔｈ…     

Isozymes Analysis of the Golden Cuttlefish Sepia esculenta (Cephalopoda: Sepiidae)

1　Introduction　ThegoldencuttlefishSepiaesculenta,aneriticanddemersalspecies,livesinthedepthof 1 0 - 1 0 0meters,andsometimesinseabottomsand .Itiswidelydistrib utedfromcentralHonshutoVietnamandthePhilip pines .Thegoldencuttlefishisalsoanimportantobjectof…     

Isozymes Analysis of the Golden Cuttlefish Sepia esculenta（Cephalopoda ： Sepiidae）

Thirty-nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP, IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram. They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good for some special enzymes, such as LDH, EST, MPI, etc. Twenty-six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao sample (P<0.99). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017, while those of the Rizhao sample are 0.023 and 0.025 respectively.     

Genetic differentiation withinEriochoir sinensis (milne, edwards) revealed by allozyme and RAPD

We analyzed 17 allozymes, and 20 primers in order to detect the genetic differentiation between commercial populations (Changjiang River, Liaohe River) ofEriochoir sinensis. Ten allozymes (LDH, MDH, ME, IDH, EST, ALP, AAT, CTL, POD, SOD) showed 21 loci by vertically discontinuos buffer system polyacrylamide gel electrophoresis. RAPD profiles generated by 12 ten-base primers showed 63 loci. The percentage of polymorphic loci and the expected heterozygosity obtained by using allozyme analysis were lower than those obtained by RAPD. The index of similarity between these two populations were 0.955 and 0.932 as revealed by allozyme analysis and RAPD technology. There was gene flow between the above populations. Contribution No. 4025 from the Institute of Oceanology and Limnology, Chinese Academy of Sciences. Project 39470141 supported by NSFC. Work also supported by Climbing Program B (PDB 6-5-3) and No. 863 High-technology Program 863-819-01-05.