Estimated respiration rate of myctophid fish from the enzyme activity of the electron-transport-system

Whole animal respiration rates (R) of myctophid fishes which migrate up to the surface at night were estimated using enzyme activities of the electron-transport-system (ETS). The fish, currently unsusceptible to laboratory experimentation, were caught at sea and stored frozen at –20°C for 14–17 days prior to enzyme assay. Supplemental tests on two tropical marine fishes (gobies and poma-centrids) showed no measurable loss of ETS activity during storage for up to 36 d at –20°C. The ETS/R ratio for gobies and pomacentrids was 1.61. Respiration rates of myctophid fishes estimated using this ETS/R ratio ranged from 17.7 to 453µl O₂ individual^–1 hr^–1 for specimens weighing 26–1101 mg wet weight atin situ temperature of 24–27°C. The relationship between the respiration rate standardized to a temperature of 20°C (R:µl O₂ individual^–1hr^–1) and wet weight (WW: mg) of myctophid fishes was expressed asR=0.790 WW^0.84 (r=0.964,n=27). This relationship does not differ appreciably from the respiration rates of other marine fishes calculated from Winberg's equation.     

Primary productivity in the fast ice area near syowa station,Antarctica, during spring and summer 1983/84

In situ measurements of the primary productivity of ice algae and phytoplankton were carried out in the fast ice area near Syowa Station (69°00S, 39°35E) during the austral spring and summer of 1983/84. Standing stock of ice algae reached a maximum of 45.1 mg chla m^–2 in late October. Phytoplankton standing stock attained a value of 3.57 mg chla m^–2 in mid-January. Primary production of ice algae in late October (7.64 mgC m^–2 hr^–1) was 14 times greater than that in mid-January (0.54 mgC m^–2 hr^–1). Production in the water column in mid-January (3.46 mgC m^–2 hr^–1) was 50 times greater than that in late October (0.07 mgC m^–2 hr^–1). These results indicate a substantial production by ice algae in the spring and by phytoplankton in the summer period.     

Seasonal and depth-related dynamics of prokaryotes and viruses in surface and deep waters of the northwestern Mediterranean Sea

The study site located in the northwestern Mediterranean Sea was visited nine times in 2005–2006 to collect water samples from the epi- (5 m), meso- (200, 600 m), and bathypelagic (1000, 2000 m) zone. Total abundance of prokaryotes and viruses was determined by flow cytometry (FCM). Prokaryotic abundance in the epi-, meso-, and bathypelagic varied between 0.9 and 15.9×10⁵, 0.6 and 2.1×10⁵, and 0.3 and 1.3×10⁵ ml⁻¹, respectively. Variation of viral abundance in the epi-, meso-, and bathypelagic was between 1.2 and 57.5×10⁶, 0.5 and 3.5×10⁶, and 0.4 and 1.3×10⁶ ml⁻¹, respectively. The fraction of low (LNA) and high (HNA) nucleic acid prokaryotes averaged 42.9% and 57.1% throughout the water column and did not differ between depth layers. Throughout the water column the fraction of low, medium, and high fluorescent viruses (Vir-LF, Vir-MF, Vir-HF) averaged 66.3%, 30.2%, and 3.5%. Vir-LF and Vir-MF did not differ between depth layers; however, Vir-HF showed a preference for surface waters. The fraction of LNA cells decreased in the epi- and increased in the bathypelagic with decreasing stratification. The fraction of Vir-LF viruses increased in the epipelagic and decreased in the bathypelagic with increasing prokaryotic abundance. Also, the relationship between viral abundance and the bacterial community was different in surface and deep waters. The data suggest that different mechanisms of interaction between viruses and their prokaryotic hosts prevail at the surface and in deep waters.     

Degradation of crude oils byPseudomonas sp. in enriched seawater medium

Microbial degradation of five crude oils such as Arabian light, Berri, Murban, Khafji and Minas crude oil by a pure bacterial strain,Pseudomonas sp. isolated from the sea water sampled at Kawasaki Harbor in Tokyo Bay, was studied experimentally in the enriched seawater medium.The degradation of crude oils was determined in total residual oil and in four fractions of saturated, aromatics, asphaltene and column residue by use of the column chromatography with activated alumina.The saturated fraction was shown to be most biodegradable. The aromatics followed for all five crude oils examined and the asphaltene was biodegradable to some extent. The column residue was not apparently degraded byPseudomonas sp. within 30 days. Each of Arabian light, Berri or Murban crude oil was degraded from 59 to 63.5 %, Khafji crude oil 49 % and Minas crude oil, solid at room temperature, only 33 %. Degradation rate of the five crude oils was determined to be in a range from 2.88 to 17.3 mg-oil 1^–1 hr^–1 or from 6.0×10^–12 to 1.56×10^–10 mg-oil cell^–1 hr^–1. Relative degradation ofn-paraffins of different carbon numbers in the saturated fraction was found to be similar regardless of carbon number for the five crude oils.     

Pelagic chaetognaths in Sagami Bay and Suruga Bay,Central Japan

Pelagic chaetognaths in Sagami Bay and Suruga Bay, Central Japan, were studied. Their community structure was very similar in both of these bays. Four genera and 26 species were identified. In Sagami Bay the habitat segregation by depths was clearly obtained. The population and species number of epipelagic (0–200 m) chaetognaths were larger in summer than in winter. The populations of meso- (200–500 m) and bathypelagic (500–1,000 m) species were smaller than that of epipelagic ones and fairly stable seasonally.     

Ammonium uptake kinetics and interactions between nitrate and ammonium uptake inChattonella antiqua

Ammonium uptake kinetics and interactions between nitrate and ammonium uptake were examined inChattonella antiqua. After the addition of ammonium to the culture ofC. antiqua, the ammonium concentration decreased linearly with time. The ammonium uptake rate as a function of ammonium concentration followed the Michaelis-Menten equation; the maximal uptake rate was 2.0 pmol cell^–1hr^–1 and the half saturation constant, 2.2M. Although the ammonium uptake was not affected by nitrate, uptake of nitrate was rapidly (15min) suppressed by ammonium and a 50% reduction in nitrate uptake was observed at an ammonium concentration ofca. 2M.     

Full-depth profiles of prokaryotes,heterotrophic nanoflagellates,and ciliates along a transect from the equatorial to the subarctic central Pacific Ocean

Studies in epipelagic waters report higher heterotrophic microbial biomass in the productive high latitudes than in the oligotrophic low latitudes; however, biogeographical data are scarce in the deep ocean. To examine the hypothesis that the observed latitudinal differences in heterotrophic microbial biomass in the epipelagic zone also occur at depth, abundance and biomass of heterotrophic prokaryotes, nanoflagellates (HNF), and ciliates were determined at depths of 5–5000 m in the central Pacific between August and September of 2005. Heterotrophic microbial biomass increased from the tropical to the subarctic region over the full water column, with latitudinal differences in prokaryotic biomass increasing from 2.3-fold in the epipelagic zone to 4.4-fold in the bathypelagic zone. However, the latitudinal difference in HNF and ciliate biomass decreased with depth. In the mesopelagic zone, the vertical attenuation rate of prokaryotic abundance, which was calculated as the linear regression slope of log-log plot of abundance versus depth, ranged from –0.55 to –1.26 and was more pronounced (steeper slope) in the lower latitudes. In contrast, the vertical attenuation rate of HNF in the mesopelagic zone (–1.06 to –1.27) did not differ with latitude. In the subarctic, the attenuation rate of HNF was 1.7 times steeper than for prokaryotes. These results suggest the accumulation of prokaryotes in the deep subarctic Pacific, possibly due to low grazing pressure. Although the vertical attenuation rate of ciliates was steepest in the bathypelagic zone, HNF abundance did not further decrease at depths below 1000 m, except for at 2000 m where HNF was lowest across the study area. Ciliate abundance ranged 0.3–0.8 cells l^–1 at 4000 m, and were below the detection limit (<0.1 cells l ^–1) at 5000 m. To our knowledge, this study presents the first data for ciliates below 2000 m.     

Estimation of Oxygen Consumption Rate Using T-DO Diagram in the Benthic Layer of Ohmura Bay, Kyushu, Japan

We have observed the temporal variation of oxygen deficient water with short time scale (less than a few days) in the central area of Ohmura Bay, Kyushu, Japan, in summer, 1995 and 1996. The vertical profiles of temperature were similar to those of dissolved oxygen. We noticed a linear relation between temperature and dissolved oxygen in the bottom layer, and applied the T-DO relation to estimate the net oxygen consumption rate, rather than direct evaluation of the advection and diffusion. Oxygen consumption rate just above the bottom was estimated to be about 0.21 g O₂ m^–3day^–1 in July 1995, and about 0.28 g O₂ m^–3day^–1 in August 1996. The net oxygen consumption rate estimated for the bottom layer below the second thermocline was about 0.61 g O₂ m^–3day^–1 with variability from 0.55 to 0.66 g O₂ m^–3day^–1 during July 25 to 29, 1995. This is was about 0.64 g O₂ m^–3day^–1 with variability from 0.18 to 1.4 g O₂ m^–3day^–1 during August 22 to 30, 1996. The net oxygen consumption rates are about half of those measured with a closed system in the Seto Inland Sea.     

Chemical environment for red tides due toChattonella antiqua

Environmental parameters that affect the growth ofChattonella antiqua were monitored throughout the outbreak period of this species around the Ie-shima Islands, the Seto Inland Sea, in the summer of 1987 (20 July–13 August). Averaged cell concentration ofC. antiqua over the water column (21 m) was below 10 cells· ml^–1 on 20 July, gradually increased to reach the maximum of 250 cells·ml^–1 on 7 August, and then rapidly decreased to the value of 30 cells·ml^–1 on 13 August.Thermal stratifications were prominent from 20 July to 3 August and were destroyed after 4 August. Temperature and salinity were optimum for the growth ofC. antiqua throughout the survey period.At the bloom initiation period (20–21 July), concentrations of N- and P-nutrients (S _N andS _P ) were high throughout the water column. From 22 July to 3 August, whenC. antiqua increased its populations,S _N andS _P at the depth of 0–5m were low but those at the depth of 10–20m kept a high value. After 4 August,S _n andS _P at the depth of 10–20m decreased rapidly due to wind mixing coupled with the nutrient uptake byC. antiqua. When the populations ofC. antiqua reached the maximum (7–9 August), N-nutrients were depleted throughout the water column but P-nutrients were not. Concentrations of vitamin B₁₂ were almost in the same range as those of the previous years and were optimum for the growth ofC. antiqua.GP- value (growth potential of the seawater with respect to nitrogen and phos-phorus) was higher than 0.6 even at the surface layer (0–5 m) at the bloom-initiation period. During the bloom development period (22 July–3 August), GP at the surface layer (0–5m) was low (<0.2), but GP at the depth of 10–20m kept a rather high value (>0.4).In situ growth rates ofC. antiqua at the depth of 0 and 5m estimated from bottle experiments coincided well with the values expected from GP. A high value of GP at the surface layer in the initiation period and a shallow GP-cline in the development period, combined with the ability of diurnal vertical migration seemed to be at least one reason that natural populations ofC. antiqua grew at a rather high rate and formed red tides in the summer of 1987.     

Investigation of Cd, Cu, Ni and Th in the colloidal size range in the Gulf of Maine

Size-fractionated seawater samples were collected from the Gulf of Maine to determine the fraction (f_c/d) of total dissolved (< 1 μm) Cd, Cu, Ni and ²³⁴Th in the colloidal size range (1,000 nominal molecular weight, NMW, to 1 μm) using cross-flow filtration. Colloidal Cd, Cu and Ni represents < 1–7% of the total dissolved concentration in these shelf waters and increases with an increase in particle concentration. By comparison, results obtained for particle-reactive ²³⁴Th indicate that < 1–47% of total dissolved is associated with the colloidal size fraction. A revised relationship between the concentration of colloids (C_c) and suspended particles (C_p) is reported (log C_c = 0.66 log C_p −2.01 kg L⁻¹) and used to examine the dependence of f_c/d for these metals on the concentration of suspended particles for C_p = 0.01–100 mg L⁻¹. Results indicate that a significant fraction (˜ 10–30%) of Cd, Cu, Ni and ²³⁴Th in the traditionally defined ‘dissolved’ fraction may exist in the colloidal size range in regions characterized by high particle concentrations (C_p > 1–10 mg L⁻¹), such as in near-shore and estuarine waters.     

Beggiatoa in microbial mats at hydrocarbon vents in the Gulf of Mexico and Warm Mineral Springs,Florida

Microbial mats were collected from a variety of sites near hydrocarbon vents along the slope in the northern Gulf of Mexico and, for comparison, from Warm Mineral Springs, Florida, USA. A predominant microorganism in each of the mats was the giant bacterium,Beggiatoa. Diameters of the bacterial filaments ranged from about 6 µm to approximately 200 µm. The latter organisms are the largest prokaryotic organisms yet found. All filaments over about 10 µm in diameter contained a large central vacuole, producing a cell with the cytoplasm as a cylindrical tube underlying the cytoplasmic membrane. Sulfur globules were confined to this peripheral layer. Push cores often contained pyrite tubules whose appearance is suggestive of aBeggiatoa origin. Determinations of ¹³C inBeggiatoa mats from vents along the Louisiana slope yielded values in the range of –26.6 to –27.9 (PDB), suggesting an unusually high degree of isotope fractionation (–24.9) relative to the carbon source in the ambient seawater, which is typical of sulfur-oxidizing chemoautotrophs. The presence of S^O (elemental sulfur) within cells ofBeggiatoa resulting from oxidation of H₂S supports the importance of bacterial sulfate reduction processes in the underlying vents for the sustenance of theBeggiatoa mats.     

Life history ofEuphausia similis (crustacea,euphausiacea) in Sagami Bay,central Japan

The life history ofEuphausia similis G.O. Sars in Sagami Bay, central Japan, has been studied by examining the abundance, size distribution, and occurrence of each growing stage from egg to adult. Three cohorts were laid within the period from July 1979 to July 1980. Individuals of cohort I of 1980 were laid in November and December 1979, and matured and spawned in March and April of the following year. Those of cohort II of 1980 laid in January and February 1980 should mature in November and spawn after December, by analogy with cohort II of 1979. Cohort III of 1980 was laid in April 1980, but decreased in number in July. Cohort II was the dominant cohort in Sagami Bay throughout the period studied. From December 1979 to April 1980 when the mixing layer was present, many eggs ofE. similis were in the epipelagic zone. It is suggested that the spawning season ofE. similis roughly coincided with the season of active feeding, indicated by high fullness of stomachs in individuals. The production of juveniles and adults was about 1.33 mg C m^–2 day^–1, and the production to bio mass (P/B) ratio was estimated to be 4.78 between July 1979 and July 1980.     

Rates of calcium carbonate dissolution and organic carbon decomposition in the North Pacific ocean

Recent carbonate data collected in the North Pacific were combined with the data in the literature in order to understand more clearly the carbonate chemistry in the North Pacific. Our analyses show that inorganic CaCO₃ dissolution contributes about 26% of the total inorganic CO₂ increase of deep water, after leaving the Southern Ocean. The calcium and alkalinity data indicate a CaCO₃ dissolution rate of 0.060±0.010 and 0.053±0.005 µ mol kg^–1 yr^–1 respectively, for waters deeper than 2,000 m in reference to the Weddell Sea Deep Water. The organic carbon decomposition rate is 0.107±0.012 µ mol kg^–1 yr^–1 while the oxygen consumption rate is 0.13±0.002 µ mol kg^–1 yr^–1. These results which are based on the direct comparison of two water masses agree well with other estimates which are based on methods such as the one-dimensional-diffusion-advection model. Comparison of data along the two sections at 165°E and 150°W shows no significant difference in the ratio of the CaCO₃ dissolution rate and the organic carbon decomposition rate. The eastern section, however, has a higher TCO₂ input than the western section because of the older age of the deep water along the eastern section.     

Vertical mixing in the bottom mixed layer near the continental shelf break in the East China Sea

The strength of the vertical mixing in the bottom mixed layer near the continental shelf break in the East China Sea was directly measured with the Micro-Scale Profiler (MSP). It has been shown that there is no significant statistical relation between the turbulent energy dissipation and the degree of the stratificationN ². It seems that the vigorous turbulence occurs not constantly but intermittently in the bottom mixed layer so that a large variation of is found depending on the time. In contrast to , the coefficient of the vertical eddy diffusivityK _z is mostly determined byN such thatK _z is large in the bottom mixed layer and small in the thermocline. Large value ofK _z in the bottom mixed layer is also found in the time series ofK _z estimated in terms of Richardson number calculated from the data obtained with electromagnetic current meters. The value ofK _z more than 10 cm²s^–1 frequently occur in the layer of 20–25 m thick just above the bottom.     

Species and concentrations of selenium and nutrients in Tanabe Bay during red tide due toGymnodinium nagasakiense

A red tide due toGymnodinium nagasakiense was observed in August 1988 in Tanabe Bay, Wakayama Prefecture, Japan. The maximum cell concentration ofG. nagasakiense reached 1×10⁵ cells ml^–1 at the surface water. From May to September 1988, the following were monitored: water temperature, salinity, chlorophylla, D.O., dissolved nutrients (NO₂–N, NO₃–N, NH₄–N, PO₄–P DON, DOP), particulate nutrients (PON, POP) and three dissolved selenium species [Se(IV), Se(VI), Organic Se]. Dissolved inorganic nitrogen (NO₃–N, NH₄–N) decreased but PON, POP, DON, DOP and inorganic phosphate increased at the peak of the bloom. The concentration of organic selenium increased up to the bloom initiation period which started on 5 July, and then the concentration of Se(IV) increased as the concentration of organic selenium decreased at the peak of the bloom (3 August). The strong relationship was found between the concentration of Se(IV) and the cell concentration ofG. nagasakiense (r ²=0.98). The Se(IV) requirement ofG. nagasakiense was 2.89×10^–17 moles cell^–1, which was agreed well with 4.4×10^–17 moles cell^–1 found in a laboratory experiment onG. nagasakiense using selenium spiked artificial sea water medium. The average ratio of Se(IV) to dissolved inorganic nitrogen (DIN) during the red tide bloom was 11441, the ratio of Se(IV) to DIN at the surface with the maximum cell concentration ofG. nagasakiense of 1×10⁵ cells ml^–1 was 1137. These results suggested that selenium may play an important role in red tide outbreak ofG. nagasakiense.     

Seasonal appearance ofProchlorococcus in Suruga Bay,Japan in 1992–1993

Seasonal appearance ofProchlorococcus was studied by flow cytometry in Suruga Bay, Japan in 1992–1993.Prochlorococcus cells were in high concentrations (>1×10⁴ cells ml^–1) from July to October 1992 and September 1993, when the water temperature was over 20°C. The 16S rRNA of the isolated cells showed 98.5% sequence homology with that ofP. marinus (Sargasso strain), indicating that they are the same species. The former has a high divinyl-chlorophyll (DV-Chl.)a/b ratio similar to the Mediterranean strain and different from the Sargasso strain. Maximum concentration ofProchlorococcus at the surface water was 2.5×10⁴ cells ml^–1 in August 1992 and their DV-Chl.a accounted for 4.0% of the total chlorophylla. A decrease in cell density to less than 5×10³ cells ml^–1 was observed from December to May with an exceptional rise in January 1993. WhileProchlorococcus showed a maximum concentration of 3.6×10⁴ cells ml^–1 at 10 m depth in September 1992, phycoerythrin (PE)-richSynechococcus spp. were dominant with their maximum concentration of 2.2×10⁵ cells ml^–1 in the same water body. On the other hand, phycocyanin (PC)-richSynechococcus spp. and the larger phytoplankters showed maximum concentrations in the surface waters in May and June. BothProchlorococcus and PE-richSynechococcus showed their lowest concentrations in April. A significant positive correlation was obtained between cell concentrations of the PE-richSynechococcus andProchlorococcus.     

Determination of the surface temperature and the temperature drop in the skin layer from a moving vessel using IR data

In this paper methods are given of the IR radiometric determination of the temperature of the radiating oceanic layer,T _s, and the determination on its basis of the temperature drop in the skin layer,dt, from on board a ship underway, which allows for the emissivity of the sea surface and the contribution of the atmosphere to the upward radiation of the sea surface. The seawater temperatureT _w was registered by a contact sensor towed along the ship at a depth of 0.1–0.4 m. The valuedt=T _s–T _w was found to be within the limits of 0.07 to –0.85 K, being on average 0.33 K. The near-surface wind speedv (v=1–13 m s^–1) had the best relationship withdt;dt=–0.66+0.06v. The empirical values ofdt were compared with the model ones obtained by the well-known formulae for the conditions of forced and free convection of the surface layer.Translated by Mikhail M. Trufanov.     

Transfer of60Co from midwater squid to sperm whales

Sperm whales are notable squid-eaters. They feed mainly on medium to large-sized cephalopods at midwater levels and defecate near the surface. This suggests the existence of an upward transport of⁶⁰Co by sperm whales from the mesopelagic zone (150–1,200 m). To elucidate this squid-whale route for this artificial radionuclide,⁶⁰Co content was determined in squid and in predator whales captured by commercial whaling. In the Cephalopoda livers⁶⁰Co levels of 30–500 mBq kg^–1 wet were found and in the viscera of Odontoceti (toothed whales) 15–40 mBq kg^–1 wet. About 0.3% of⁸⁰Co ingested was estimated to be retained in a 23-year-old male sperm whale. In the livers of Bryde's whales,⁶⁰Co levels of 40–80 mBq kg^–1 wet were detected, but not in euphausiids and sardines, their possible prey. The level of Co in sperm whales was nearly the same as in Bryde's whales. Specific radioactivity⁶⁰Co/⁵⁹Co in mBq µg^–1 was several times higher in sperm whale (1.1–1.6) than in cephalopods (0.19–0.77). Eating prey with a high content of⁶⁰Co in the 1960's may have contributed to the present body burden in sperm whales with a long-life span. However, the origin of⁶⁰Co in Bryde's whales is unknown.     

Phosphorus content of zooplankton from the Inland Sea of Japan

Measurements of phosphorus content were made for eleven species of copepods, two species of mysids, shrimps (including mysis stage) and chaetognaths, and one species ofNoctiluca, cirriped (naupliar stage) and crab (zoeal stage) from the Inland Sea of Japan. The body phosphorus content (P, inµg) is highly correlated to prosome length (L, inµm) for copepods, which is expressed as logP=2.90 ×logL–9.34. Mean phosphorus content expressed as percent of dry weight is 1.30% for copepods, 1.39% for mysids, 0.63% for a decapod shrimpAcetes japonicus, and 0.80% for chaetognaths.Noctiluca contains phosphorus of only 0.36%. The comparatively higher phosphorus content of suspension-feeding copepods may be related to the constant supply of phosphorus-rich phytoplankton in this region.     

Feeding chronology of the vertically migrating gonostomatid fish,Vinciguerria nimbaria (Jordan and Williams), off southern Japan

The feeding habits of the gonostomatid fish,Vinciguerria nimbaria (Jordan andWilliams), from off southern Japan were studied in relation to its food organisms, process of digestion, and diel vertical migration. Food organisms were composed mainly of small- to moderate-sized copepods which live almost entirely in the 0–200 m epipelagic zone.V. nimbaria appears to feed twice a day; after having migrated to the epipelagic zone in the evening, all individuals fed intensively from 6 to 8 p.m. They then spent the remainder of the night in digestion. Many, but not all, of the individuals foraged again in the morning.