H'、AMBI和M-AMBI指数在黄河口及邻近海域底栖生境健康评价中的适宜性研究

From a dataset of macrobenthos obtained from 18 cruises from 2004 to 2013 in the Huanghe(Yellow River)Estuary and its adjacent areas,the composition and characteristics of macrobenthos were analyzed,and the applicability of the Shannon-Wiener Index(H′),AZTI's Marine Biotic Index(AMBI) and multivariate AMBI(MAMBI) for assessing benthic habitat quality was compared.The results showed a total of 203 macrobenthos in the study area.The macrobenthos were dominated by polychaetes,followed by mollusks and crustaceans.The macrobenthic ecological groups were dominated by EGI,EGII and EGIII,which respectively accounted for 31.5%,36.0% and 21.2% of the total.There were significant differences between the evaluation results of the three indices.The ecological quality status(EQS) levels given by the AMBI were greater than those given by the H′ and M-AMBI.The AMBI could not reflect the differences between 11 sites but the H′ and M-AMBI could do.Moreover,the three indices responded well to the variations in salinity(S) and dissolved oxygen(DO) in the waters.The H′ and MAMBI also responded sensitively to the differences in physical parameters,such as water depth and sediment texture.The correlation between M-AMBI and environmental pressure gradient data was the strongest.The MAMBI could effectively distinguish degraded conditions from undegraded but the H′ and AMBI could not.Therefore,the M-AMBI reflected benthic habitat health well in the study areas.However,the objectivity of evaluation results of M-AMBI needs further verification by physical,chemical and biological methods.The thresholds also need further discussion.     

受扰动海域大型底栖动物群落特征及基于AMBI和M-AMBI指数的生态健康评价：以山东烟台辛安河口为例

To understand the ecological status and macrobenthic assemblages of the Xin'an River Estuary and its adjacent waters, a survey was conducted for environmental variables and macrobenthic assemblage structure in September 2012(Yantai, China). Several methods are adopted in the data analysis process: dominance index,diversity indices, cluster analysis, non-metric multi-dimentional scaling ordination, AMBI and M-AMBI. The dissolved inorganic nitrogen and soluble reactive phosphorus of six out of eight sampling stations were in a good condition with low concentration. The average value of DO((2.89±0.60) mg/L) and p H(4.28±0.43) indicated that the research area faced with the risk of ocean acidification and underlying hypoxia. A total of 62 species were identified, of which the dominant species group was polychaetes. The average abundance and biomass was577.50 ind./m2 and 6.01 g/m2, respectively. Compared with historical data, the macrobenthic assemblage structure at waters around the Xin'an River Estuary was in a relatively stable status from 2009 to 2012.Contaminant indicator species Capitella capitata appeared at Sta. Y1, indicating the animals here suffered from hypoxia and acidification. AMBI and M-AMBI results showed that most sampling stations were slightly disturbed,which were coincided with the abiotic measurement on evaluating the health conditions. Macrobenthic communities suffered pressures from ocean acidification and hypoxia at the research waters, particularly those at Stas Y1, Y2 and Y5, which displays negative results in benthic health evaluation.     

基于多种生物指数的中国渤海莱州湾海域底栖生境健康评价

As an important part of the Bohai economic rim, the Laizhou Bay has been stressed by serious eco-environmental problems in recent years. In this study, the Shannon-Wiener index(H′), AZTI's marine biotic index(AMBI) and the multivariate AMBI(M-AMBI) were used to assess the ecological quality status(EQS) of the Laizhou Bay according to macrobenthos data collected annually in August 2011–2014. The results showed that the overall benthic habitat quality in the Laizhou Bay was assessed as "Good". However, 25% of the samples were classified as"Moderate", "Bad" or "Poor" status under degraded conditions. Ecological group Ⅲ(EGⅢ) species which had a certain tolerance to environmental disturbances had a higher proportion in each station, and most of them had appeared the pollution indicator species Capitella capitata. This indicated that the benthic habitat in the Laizhou Bay had been disturbed and polluted to some extent. The comparison of the three indices evaluation result and the RDA analysis showed that the H′ and M-AMBI were more suitable when the relative abundance of the single species was high and the macrobenthic community was significantly imbalanced; when the relative abundance of opportunistic species(EGIV and EGV) was high, the AMBI and M-AMBI could reflected the EQS objectively; in an undisturbed and polluted environment, all the three indices could indicate the benthic habitats quality. In summary, the better correlation between the three indices and environmental factors showed that they were well responsive to the tendency of the benthic habitats quality in the Laizhou Bay.     

Assessing the benthic quality status of the Bohai Bay (China) with proposed modifications of M-AMBI

Multivariate AZTI's Marine Biotic Index (M-AMBI) was designed to indicate the ecological status of European coastal areas. Based upon samples collected from 2009 to 2012 in the Bohai Bay, we have tested the response of variations of M-AMBI, using biomass (M-BAMBI) in the calculations, with different transformations of the raw data. The results showed that the ecological quality of most areas in the study indicated by M-AMBI was from moderate to bad status with the worse status in the coastal areas, especially around the estuaries, harbors and outfalls, and better status in the offshore areas except the area close to oil platforms or disposal sites. Despite large variations in nature of the input data, all variations of M-AMBI gave similar spatial and temporal distribution patterns of the ecological status within the bay, and showed high correlation between them. The agreement of new ecological status obtained from all M-AMBI variations, which were calculated according to linear regression, was almost perfect. The benthic quality, assessed using different input data, could be related to human pressures in the bay, such as water discharges, land reclamation, dredged sediment and drilling cuts disposal sites. It seems that M-BAMBI were more effective than M-NABMI (M-AMBI calculated using abundance data) in indicating human pressures of the Bay. Finally, indices calculated with more severe transformations, such as presence/absence data, could not indicate the higher density of human pressures in the coastal areas of the north part of our study area, but those calculated using mild transformation (i.e., square root) did.     

夏季渤海西南部及黄河口海域营养盐分布特征

There are few papers dealing with nutrients distribution in the mixing region of the Huanghe River Estuary, although the Huanghe River is one of the largest rivers in China. We investigated nutrients (nitrate nitrite, phosphate and silicate) at 81 stations in the southwest Bohai Sea and the Huanghe River Estuary in July, 1984. The concentrations of nitrate, nitrite, phosphate and silicate are higher in the estuary and offshore than those in the centre of the Bohai Sea. They are 44.3ug-at/L for nitrate, 0.31ug-at/L for nitrite, 0.36ug-at/L for phosphate and 60.4ug-at/L for silicate. There are negative correlations between nutrients and salinity in this region. The correlation coefficients in surface layer are:γSiO3-si=-0.963(n=81) γPO4-P=-0.829(n=81) γNO3-N=-0.971(n=81) γNO2-N=-0.553(n=81) The correlation coefficients show that, in the surface layer, there are no obvious removal of nutrients during mixing river-water and seawater in the Huanghe River Estuary.     

NOTES

Based on the data of four seasonal surveys in 1980 and 1981, this paper analyses, forthe first time, the structure and distribution of benthic fauna and the relationship betweenbenthic fauna and the environmental factors. The results show that the benthic fauna com-munities in the survey area are quite complicated with eight community distribution areas,three ecological community types and four community structure types. The obvious com-     

Chemometric study of spatial variations of environmental and ecological characteristics in the Zhujiang River (Pearl River) Estuary and adjacent waters

Chemometric approach based on principal component analysis (PCA) was utilized to examine the spatial variances of environmental and ecological characteristics in the Zhujiang River (Pearl River) Estuary and adjacent waters (ZREAW) in the South China Sea. The PCA result shows that the ZREAW can be divided into different zones according to the principal components and geographical locations of the study stations, and indicates that there are distinct regional variances on environmental features and the corresponding phytoplankton biomass and community structures among different areas. The spatial distribution of ecological features was implied to be influenced by various degrees of the different water resources, such as the Pearl River discharges, the coastal current and the oceanic water from the South China Sea. The variation of the biomass maximum zone and the complex impacts on the spatial distributions of phytoplankton biomass and production were also evaluated.     

THE STRUCTURE AND HEAT BUDGETS OF LARGE-SCALE DISTURBANCES OVER THE EQUATORIAL TROUGH REGION OF THE WESTERN PACIFIC

The average vertical distribution of equivalent potential temperature, horizontal divergerce, relative vorticity and vertical velocity overthe observation area were computed using 6-days NAVAID wind data from the R/V Shijian and R/V Xiangyanghong 09 during FGGE (the First GARP Global Experiment) and upper air sounding data of two island stations within the same period. Yanai's method was used to compute the vertical distributions of the apparent heat source, apparent moisture sink and total eddy heat flux. Satellite cloud photographs and surface meteorological measurements were used to subdivide the six days of observation into three disturbed days and three undisturbed or trade days. This paper describes the large-scale structure and heat budgets in the disturbed and trade wind periods and compared them with those of the trade wind regions of the Atlantic. Some interesting results of the structure and heat budgets of large-scale disturbances over the equatorial trough region in the western Pacific are     

Fish Faunas in the Southern Waters of the Taiwan Strait and Its Adjacent Areas

Fish species are abundant in the southern waters of the Taiwan Strait and its adjacent areas where 678 species in all are presnt, mostly belonging to 18 families such as Carangidae, Sciaenidae, Serranidae, Goboodae and others. In the composition of species, 73% of them are those distributing in the Indo-Malayan Region and the tropical waters of the Indo-Pacific Region. The fish faunas have greater similarity to those in the northern waters of the South China Sea, 96% being in common. Species     

The grain-size distribution of the suspended particulate matter in the Huanghe Estuary and its adjacent sea area in winter

The grain-size of suspended particulate matter (SPM) in the Huanghe Estuary and its adjacent sea area was investigated with an in situ laser particle size analyzer (LISST-100) in November 2006.The spatial distribution of the grain-size parameters was very complicated.The results show that (1) the mean particle size of SPM ranged from 3.00Φ to 6.41Φ,with an average value of 4.66Φ;(2) the frequency distribution patterns of the SPM grain-size showed three different types,which were mono-mode,dual-mode and tri-...     

Preliminary results of biological monitoring using benthic macroinfauna of the discharge areas of Lisbon drainage basins in Tagus estuary after new developments in sanitation infrastructures

The present study focused on the use of benthic macroinfauna to assess the ecological quality status of two specific areas in the Tagus estuary (Portugal), after the improvement in the sanitation infrastructures discharging in those areas. The sampling campaigns were held in summer of 2009, 2010 and 2011 in a set of impact and control stations. Multivariate analysis was used to determine the evolution of spatial and temporal patterns in benthic macroinfauna assemblages' composition. The ecological status of these assemblages was assessed by means of taxa richness, Shannon–Wiener diversity index and AMBI and M-AMBI biotic indices. The results obtained in both study areas seemed to indicate a trend for an improvement in the ecological condition of their benthic macroinfauna assemblages over time, although a consistent pattern was not found in all the analyses performed. When benthic macroinfauna assemblages under analysis are considerably degraded, the multimetric index M-AMBI seems to be more adequate for conducting monitoring studies than the AMBI index. However, the continuation of this work is indispensable to ensure the validity of this conclusion.     

基于AMBI和M-AMBI法的底栖生态环境质量评价——以厦门五缘湾海域为例

于2011-2013年5月和10月在厦门五缘湾海域设置7个站位进行大型底栖动物调查,并结合2005年的历史数据,运用AMBI和M-AMBI指数法评价该海域海底生态环境质量的时空变化状况。2011-2013年共鉴定大型底栖动物9门106科193种,多毛类(83种)、甲壳类(53种)和软体动物(25种)是该海域的主要优势类群,平均总密度和湿质量生物量分别为(518±292)ind/m2和(54.8±88.9)g/m2。多毛类的短鳃树蛰虫(Pista brevibranchia)为该海域的绝对优势种,其平均密度可达(170±179)ind/m2,其他优势种还有似蛰虫(Amaeana trilobata)、西方似蛰虫(Amaeana occidentalis)、丝鳃稚齿虫(Prionospio malmgreni)、梳鳃虫(Terebellides stroemii)、独毛虫属一种(Tharyxsp.)、菲律宾蛤仔(Ruditapes philippinarum)、长指马尔他钩虾(Melita longidactyla)和伍氏蝼蛄虾(Austinogebia wuhsienweni)等。评价结果显示,该海域底栖生态系统受轻度干扰,生态环境质量介于一般—优良之间,与2005年的调查相比,2011-2013年该海域的生态环境质量有所好转,这可能与五缘湾及周边海域近年来进行的海堤开口改造、海洋环境整治,以及海湾纳潮量及水交换能力的改善有关。     

Assessing the ecological status of the North-western Adriatic Sea within the European Water Framework Directive: a comparison of Bentix, AMBI and M-AMBI methods

In this paper we looked for the most suitable method for benthic ecological quality status (EcoQS) assessment of the North-western Adriatic Sea. Taking into account the historical and present information, we inter-compared our expectations in terms of EcoQS, with the Bentix, AMBI and M-AMBI classification metrics results, and investigated the sensitivity of these indices to the spatial, seasonal and inter-annual variability of benthic communities. The sampling sites were located at increasing distances with respect to the Po River mouth, along an (inverse) gradient of eutrophication. Moreover, a 2-year monthly monitoring plan (October 2004–September 2006) was carried out at the CEBEB site, located in a central position with respect to the investigated area. The results highlighted that the three indices differed in the sensitivity to spatial and temporal variability of the benthic communities. The strong seasonal and inter-annual changes in the benthic communities had a significant effect on the Bentix, whereas no relevant modifications were observed on the EcoQS assignation obtained using the M-AMBI and AMBI methods. The environmental quality ratios (EQR) values and EcoQS classification obtained by the Bentix and AMBI did not match expectations, as they were not inversely correlated with the site distances with respect to the Po mouth, whereas the M-AMBI clearly discriminated the most and the least eutrophicated sites. It thus appears that the M-AMBI could be considered to be the most valuable method for the ecological-status assessment of the North-western Adriatic Sea benthic ecosystem.     

基于3种生物指数的三沙湾养殖活动底栖环境效应研究

为考察我国东海区最为典型养殖海湾三沙湾养殖活动的底栖环境效应,本研究根据2016年8月航次41个样品采集站位的数据,分析香农-威纳多样性指数（Shannon-Wiener index,H'）、海洋生物指数AMBI（AZTI's Marine Biotic Index）和多变量海洋生物指数M-AMBI（Multivariate-AMBI）在4种典型水域（网箱、海藻、鲍鱼养殖和无养殖水域）间的差异及其与沉积物环境因子（δ15N、δ13C、C/N比、总有机碳、总氮、总磷、硫化物、含水率和粉粒-黏粒比例）的相关性。结果表明,航次共采集74种大型底栖生物,H'介于0~3.51;物种多属环境敏感类型（AMBI方法中EGⅠ和EGⅡ生态组）,AMBI指数介于0~4.13,M-AMBI指数介于0.30~0.77。3种生物指数的分布水平表明此海域总体底栖生态质量良好,仅在局部水域存在显著扰动。Kruskal-Wallis检验显示仅H'在不同类型水域之间存在显著差异（P<0.05）,后续配对检验（Pairwise comparision）显示大黄鱼养殖水域H'显著高于海藻养殖水域（P<0.01）。基于斯皮尔曼秩相关系数（Spearman correlation coefficient）的相关性分析表明仅H'和M-AMBI指数分别与总磷存在显著相关性（P<0.05）。聚类分析（Cluster analysis）结果显示不同类型站位在聚类图中随机分布,聚类结果未体现出采样水域的差异性。综上,基于3种生物指数的分析结果显示三沙湾内网箱等典型养殖活动未对区域底栖生境质量造成显著影响。     

Paradigmatic responses of marine benthic communities to different anthropogenic pressures, using M-AMBI, within the European Water Framework Directive

The use of AMBI and M-AMBI in benthic quality assessments, within the European Water Framework Directive (WFD), has increased dramatically in recent years. M-AMBI is a multivariate tool, which incorporates AMBI, richness and Shannon diversity within the assessment. The response of this approach to different human pressures in coastal and estuarine waters has been investigated for the Basque Country (Northern Spain). In this contribution, several paradigmatic examples of these applications to different water bodies are presented. The time-series extend to samples collected between 1995 and 2007, showing the evolution of M-AMBI values under different pressures, i.e. urban and industrial discharges, dredging and disposal of sediments, and engineering works (such as land reclamation or marina construction), and, in addition, for illustrating the benthic quality recovery after positive actions have been undertaken, i.e. the removal of point-source discharges or water treatment programmes. In most cases, M-AMBI responds to these pressures as expected, with decreases in the ecological status immediately following the pressure. Conversely, when a pressure is removed, the recovery takes between 2 and 15 years, depending upon the intensity of the pressure and the characteristics of the water body. M-AMBI has been intercalibrated previously in coastal waters. After intercalibration in transitional waters, M-AMBI will be able to be used in the integrative quality assessment of European water bodies.     

Spatiotemporal variations of benthic macrofaunal community in the Xiamen Amphioxus Nature Reserve, eastern South China Sea

In order to realize the spatiotemporal variations of benthic macrofaunal communities at the "Amphioxus Sand"habitat, six surveys including four seasons and three consecutive summers(i.e., 2014, 2015 and 2016) were conducted in two core sites, i.e., Huangcuo(HC) and Nanxian-Shibaxian(NX), in the Xiamen Amphioxus Nature Reserve in China. A total of 155 species of macrofauna were recorded, therein, polychaetes were dominant in terms of species number and density. Significant spatiotemporal variations of macrofaunal communities were observed. The density of polychaetes and the biomass of molluscs in the HC site were higher than those in the NX site. Macrofauna were more diverse and abundant in the cold seasons(winter and spring) than that in the warm seasons(summer and autumn). The annual variations of macrofaunal communities may be attributed to the changes in sediment texture among the three years of the survey. The variations in macrofaunal communities were mainly related to the proportion of polychaetes within the community. In addition, the density of amphioxus(include Branchiostoma japonicum and B. belcheri) was negatively correlated to that of polychaetes, bivalves, and crustaceans. Amphioxus was less likely to be found in the sediments with higher silt and clay content. Five biotic indices including Margaref's richness index(d), Peilou's evenness index(J′), Shannon-Wiener diversity index(H′),AMBI and M-AMBI were calculated in the present study. AMBI seems suitable in assessing benthic health at the"Amphioxus Sand" habitat, and a potential risk of ecological health in Xiamen Amphioxus Nature Reserve should be aware.     

黄河口海域调水调沙期间大型底栖生物群落特性研究

黄河口海域大型底栖生物群落结构受到包括自然和人为在内的诸多因子的影响,本次研究以2011年调水调沙活动对该海域大型底栖生物群落结构的影响研究为主,分析其短期内群落结构的变化。调水调沙前、中、后期均采用同样的站位取样,分别为1～18号站位,按照距离黄河入海口的距离分为4个梯级,为5、10、20和40km。研究结果显示：调水调沙前、中、后期分别渔获大型底栖生物73,72和85种,重要种的数量分别为13,1和16种;所有种类可以统计为4个门类,为环节动物、软体动物、节肢动物和棘皮动物,其中,环节动物和软体动物占比重最大,而1982年其他学者对莱州湾大型底栖生物的研究显示,棘皮动物和环节动物占比重最大,这表明近30年该海域大型底栖生物的群落结构产生较大变化;BOPA指数显示,前期各站位水质较高,中期和后期分别有2,3个站位受到中等程度污染;ABC曲线分析说明,调水调沙中、后期,大型底栖生物群落受扰动较大;群落聚类分析和冗余分析（RDA）表明,大型底栖生物群落结构与环境因子关系密切,其中,调水调沙中期有机物对群落结构的影响最大,而后期的较大影响因子为水深。环境因子并不是单一作用于生物群落,而是几个环境因子共同作用,包括有机物、硫化物、重金属Hg和As等;不同环境因子造成群落结构变动的原因较多,其一可能是海水透明度的下降造成大型底栖生物的生长和繁殖受阻。本次研究重点表明,人类多元活动从短期和长期看对大型底栖生物的群落结构变动影响均较大,调水调沙活动对黄河口海域大型底栖生物的栖息地作用明显,改变其水温、盐度、群落扰动等。因此,应当加强对该海域入海陆源污染物的控制,加强对海洋生态平衡的保护。