利用RAPD和ISSR引物对三系杂交水稻及其父母本的鉴定

为了鉴定杂交种子与父母本的亲缘关系，用20条随机引物和21条ISSR引物在供试的一组三系杂交水稻F1、父母本及对照进行扩增。20条RAPD引物共扩增出78个条带，其中28条为多态性条带；21条ISSR引物共扩增出72个条带，其中多态性条带为20条。将这些多态性条带进行聚类分析，F1与父、母亲缘关系较近归为一类．随后它们才与对照归为一类。结果表明RAPD和ISSR引物的PCR扩增均能有效地鉴定三系杂交水稻的杂种F1与组合中父、母本的遗传关系。与RAPD相比ISSR引物在三系杂交水稻及其父母本的鉴定中具有稳定性更好的优点。     

利用RAPD和ISSR引物对三系杂交水稻及其父母本的鉴定

为了鉴定杂交种子与父母本的亲缘关系,用20条随机引物和21条ISSR引物在供试的一组三系杂交水稻F1、父母本及对照进行扩增。20条RAPD引物共扩增出78个条带,其中28条为多态性条带;21条ISSR引物共扩增出72个条带,其中多态性条带为20条。将这些多态性条带进行聚类分析,F1与父、母亲缘关系较近归为一类,随后它们才与对照归为一类。结果表明RAPD和ISSR引物的PCR扩增均能有效地鉴定三系杂交水稻的杂种F1与组合中父、母本的遗传关系。与RAPD相比ISSR引物在三系杂交水稻及其父母本的鉴定中具有稳定性更好的优点。     

企鹅珍珠贝同工酶表达多态性的初步研究

采用垂直板状聚丙烯酰胺梯度凝胶电泳,对企鹅珍珠贝鳃组织的酯酶(EST)、葡萄糖-6磷酸脱氢酶(G6PD)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)5种同工酶进行了检测,并对酶谱特征进行了生化遗传分析.酶谱表型显示企鹅珍珠贝的同工酶呈现多态现象,同工酶表达上的多态性表明企鹅珍珠贝具有较丰富的遗传多样性.     

企鹅珍珠贝同工酶表达多态性的初步研究

采用垂直板状聚丙烯酰胺梯度凝胶电泳，对企鹅珍珠贝鳃组织的酯酶(EST)、葡萄糖-6磷酸脱氢酶(G6PD)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)和超氧化物歧化酶(SOD)5种同工酶进行了检测，并对酶谱特征进行了生化遗传分析。酶谱表型显示企鹅珍珠贝的同工酶呈现多态现象，同工酶表达上的多态性表明企鹅珍珠贝具有较丰富的遗传多样性。     

Genetic Variation and Differentiation in Wide Ranging Populations of Razor Clam （Sinonovacula constricta） Inferred from AFLP Markers

The genetic variation and differentiation of the razor clam Sinonovacula constricts distributed along the coast of China were studied through amplified fragment length polymorphism(AFLP)analysis. Six primer combinations generated 193 fragments. The He values varied from 0. 322 to 0. 463 and the percentage of polymorphic loci ranged from 74. 1% to 98. 4%, which indicates a high level of genetic diversity. Cluster analysis by Nei's pairwise distance grouped all specimens by geographical origins. AMOVA consistently showed that genetic variation among populations was 8. 71%, and most of the variation came from the genetic variation within populations(91.29%). Genetic differentiation among the six populations was moderate; pairwise FST ranged from 0. 0282 to0. 1480, which indicated that S. Constricts populations along the coast of China are genetically connected. Among all the six populations, the Beihai population is the mostly differentiated from the others, suggesting that Hainan Island and Leizhou Peninsula act as barriers to gene flow. All populations abide isolation by distance model as indicated by Mantel test, except for ZS(Zhoushan)and YQ(Yueqing)populations. Information obtained in this study will provide guidelines for conservation and fishery management of this species in the future.     

两个凡纳滨对虾亲本群体的RAPD研究

采用Operon公司的 16个引物对两批凡纳滨对虾亲虾的基因组DNA多态性进行了检测 ,结果表明 ,第一个亲本群体对 16个引物共检测到 78个位点 ,其中多态位点数为 4 8个 ,占 6 1.5 4% ;第二个亲本群体共获得 97个位点 ,其中多态位点数为 4 9个 ,占 5 0 .5 2 %。单个引物获得的标记数为 1～ 8个。第一个亲本群体个体间的平均遗传距离为 0 .196 0± 0 .0 392 ,第二个亲本群体个体间的平均遗传距离为 0 .0 92 2± 0 .0 189。两个亲本群体间的多态位点比例为 6 6 .98% ,遗传距离为0 345 5± 0 .0 795。在OPF 0 9、OPV 19、OPZ 113个引物的扩增结果中 ,发现两个亲本群体有明显差异。     

Development of 101 Novel EST-Derived Single Nucleotide Polymor- phism Markers for Zhikong Scallop （Chlamysfarren）

Zhikong scallop （Chlamys farreri） is an important maricultured species in China. Many researches on this species, such as population genetics and QTL fine-mapping, need a large number of molecular markers. In this study, based on the ex- pressed sequence tags （EST）, a total of 300 putative single nucleotide polymorphisms （SNPs） were selected and validated using high resolution melting （HRM） technology with unlabeled probe. Of them, 101 （33.7%） were found to be polymorphic in 48 indi- viduals from 4 populations. Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500. The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505, respectively. Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers. BLASTx gave significant hits for 72 of the 101 poly- morphic SNP- containing ESTs. Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons （33 SNPs） or pretermination of translation （1 SNP）. The markers developed can be used for the population studies and genetic improvement on Zhikong scallop.     

基于转录组测序的方斑东风螺单核苷酸多态性位点挖掘及功能注释

【目次】了解方斑东风螺中响应LPS刺激的SNP位点及SNP所在基因SNP-Unigenes的功能。【方法】使用SOAPsnp软件分析不同时间条件下转录组数据中的SNP位点,使用GO、COG和KEGG数据库进行功能注释。【结果】转录组数据中共挖掘到673 782个SNP位点,分布在110 869条SNP-unigenes序列上。SNP类型分析表明,转换位点发生的频率远高于颠换位点,且6种单碱基变异类型中以A/G转换发生概率最大。有5866条SNP-unigenes富集到298个KEGG子集中,其中以"内吞作用"子集中富集的SNP-Unigenes最多,共富集182条。     

流沙湾卵鳎微卫星标记的遗传多样性分析

为分析湛江流沙湾海域优势渔种卵鳎的遗传多样性,应用微卫星标记技术,选用15对微卫星引物,以等位基因数、基因杂合度、多态信息含量、固定指数等遗传参数为指标,评估卵鳎群体内的遗传多态性。结果表明:共检测到90个等位基因,等位基因数从1~12不等,平均为6.0;有效等位基因数从1.0~8.4,平均为4.0,多态性位点比例为53%,显示其具有中等杂合子水平,其中8个多态位点的期望杂合度(He)为0.670~0.881,平均为0.800,观测杂合度(Ho)为0.353~1.000,平均为0.773,多态信息含量(PIC)值为0.616~0.870,平均为0.773,群体内固定指数F为-0.199~0.564,平均为0.046;流沙湾卵鳎群体具有高度遗传多样性。     

猪雌激素受体(ESR)基因的多态性分析

母猪的产仔数是一个重要的经济性状 ,产仔数的提高将大大增加肉猪的产量 ,给养猪业带来巨大的经济效益 ,因此 ,有效地提高猪的产仔数一直是猪育种的主要目标之一。由于猪产仔数的遗传力很低 (约为 0 .1 ) ,并且要等到母猪产仔后才能度量 ,这种传统的选育方法其育种周期长、遗传进展小 ,因此开发和利用新的选育标记来提高猪的繁殖性就倍受重视。随着现代分子生物学和分子遗传学的发展 ,使得寻找多仔基因的遗传标记和标记辅助选择(Ｍａｒｋｅｒａｓｓｉｓｔｅｄｓｅｌｅｃｔｉｏｎ ,ＭＡＳ)成为可能。 1 994年Ｒｏｔｈｓｃｈｉｌｄ通过候选基…     

The observed currents in summer in the Bohai Sea

A harmonic method was used to analyze the tidal currents observed in summer at 11 stations made from 1996 to 2001 in the Bohai Sea, China. Data was compared among different instruments and intervals. Elliptic elements were calculated based on harmonic constants, of which vertical distributions of the maximum speed and rotation direction were discussed for understanding the characteristics of diurnal and semi-diurnal tidal current components. The results indicate that the maximum speed of M2 tidal current component is much larger than that of K1; the rotation direction of M2 tidal current constituent is clockwise in the central part of the Bohai Sea and in the Laizhou Bay, but anticlockwise in the Liaodong Bay and Bohai Bay. For K1 tidal current constituent, it is clockwise in the central Bohai Sea but anti-clockwise in the Laizhou Bay and Liaodong Bay. The tidal currents in most stations in the Bohai Sea were regular semidiurnal except for those in the central Bohai Sea, being irregular semidiurnal.     

Advance in Application of Regional Climate Models in China

Regional climate models have become the powerful tools for simulating regional climate and its change process and have been widely used in China. Using regional climate models, some research results have been obtained on the following aspects： 1） the numerical simulation of East Asian monsoon climate, including exceptional monsoon precipitation, summer precipitation distribution, East Asian circulation, multi-year climate average condition, summer rain belt and so on; 2） the simulation of arid climate of the western China, including thermal effect of the Qinghal-Tibet Plateau, the plateau precipitation in the Qilian Mountains; and the impacts of greenhouse effects （CO2 doubling） upon climate in the western China; and 3） the simulation of the climate effect of underlying surface changes, including the effect of soil on climate formation, the influence of terrain on precipitation, the effect of regional soil degradation on regional climate, the effect of various underlying surfaces on regional climate, the effect of land-sea contrast on the climate formulation, the influence of snow cover over the plateau regions on the regional climate, the effect of vegetation changes on the regional climate, etc. In the process of application of regional climate models, the preferences of the models are improved so that better simulation results are gotten. At last, some suggestions are made about the application of regional climate models in regional climate research in the future.     

Lithological indicators of discontinuities in mountain soils rich in calcium carbonate in the Polish Carpathians

Six soils located within the Polish Carpathians,developed on calcium carbonate–rich sedimentary parent materials and representing various reference groups,were investigated in order to detect the lithic discontinuity.We propose using a multidirectional approach to assess the lithic discontinuity in these soils,one that includes grain size distribution,geochemical composition,heavy mineral content and micromorphology,supported by a traditional soil survey.A further aim of this process was to identify the possible admixture of allochthonous material of aeolian origin.The studied soils presented lithic discontinuities mostly at the contact of underlying calcium carbonate–rich coarsegrained slope deposits with the overlaying colluvium layer having a lower content of rock fragments.The significant changes in grain size distribution,especially in the silt and sand content,as well as high Uniformity Values and partially,high Lithological Discontinuity Index values,confirmed the occurrence of a lithic discontinuity in all studied soils.High heterogeneity in the soil profiles was also confirmed by the distribution of the major oxides;however,their distribution did not clearly indicate the lithic discontinuity.The most visible distinctions were noted from CaO content,which resulted from the deposition of carbonate-free materials(aeolian silts)and their mixing with the calcium carbonate–rich parent material.Furthermore,the analysis of heavy mineral content confirmed the allochthonous origin of the upper(and in some cases also the middle)parts of all profiles,which was manifested by the presence of highly weathering-resistant minerals such as zircon,epidote and various types of garnets.The micromorphological features of some of the studied soils showed distinctiveness within the soil profile,manifested by changes in b-fabric pattern,the occurrence and distribution of secondary carbonate and the coarse and fine coarse and fine ratio.Based on the high content of silt within the upper and middle parts of the soils,the content of Hf and Zr,as well as the higher content of weathering-resistant minerals,admixture of aeolian silt could be considered in some of the studied soils,yet with weak character.However,the dominance of minerals typical for metamorphic and igneous rocks suggested that the supply of aeolian silt was associated with loess covers rather than local sedimentary material.     

Microsatellite DNA polymorphisms and the relation with body weight in sea cucumber <Emphasis Type="Italic">Apostichopus japonicus</Emphasis>

The relationship between microsatellite polymorphism and body weight of captive bred Chinese sea cucumber Apostichopus japonicus was investigated in two local populations in Dalian. Among ten loci discovered, nine show changes except for AJ07 loci. Seven loci were found highly polymorphic in both populations. For each locus in two populations, the average number of alleles is 6.428 6 and 6.285 7, the average observed heterozygosity at 0.225 7 and 0.245 9, the expected heterozygosity at 0.776 8 and 0.748 8, the polymorphism information content (PIC) at 0.709 2 and 0.674 6, respectively. Further analysis show significant correlation between A. japonicus body weight and occurrence markers AJ02 and AJ04. The findings of the relation may be helpful for molecular breeding, as well as the marker-assisted selection of sea cucumbers. Supported by the National High Technology Research and Development Program of China (863 Program, No. 2006AA10A411), the Natural Science Foundation of Liaoning Province (No.20072139), the Grant of Dalian Fisheries University, the Key Laboratory Foundation of the Educational Department of Liaoning Province (No.2009S024)     

斑节对虾两个野生种群RAPD分析

斑节对虾（Penaeus monodon）是世界上三大优良养殖品种之一，广泛分布于印度洋和西太平洋，也是我国本土优质对虾资源之一。但尚未形成遗传稳定的养殖品系，养殖种苗除少数来自海捕外，绝大多数通过人工繁殖获得。供人工繁殖的亲虾均来自野生资源。斑节对虾养殖的迅速发展对野生资源造成了极大的压力，过度开发势必引起种质的退化。