ENCLOSURE EXPERIMENTS ON AND LACUSTRINE PRACTICE FOR ELIMINATING MICROCYSTIS BLOOM

ＩＮＴＲＯＤＵＣＴＩＯＮＭｉｃｒｏｃｙｓｔｉｓａｅｒｕｇｉｎｏｓａｗａｓｔｈｅｍａｉｎｂｌｏｏｍ ｆｏｒｍｉｎｇｓｐｅｃｉｅｓｉｎＤｏｎｇｈｕＬａｋｅ;ｏｃｃｕｒｒｅｄｏｎｌｙｓｐｏｒａｄｉｃａｌｌｙｉｎｔｈｅ 1 950ｓ,ｉｎｃｒｅａｓｅｄｒａｐｉｄｌｙｉｎｎｕｍｂｅｒｉｎｔｈｅ 1 960ｓ.Ｆｒｏｍｔｈｅｍｉｄｄｌｅｏｆｔｈｅ 1 970ｓｔｏｔｈｅｍｉｄｄｌｅｏｆｔｈｅ 1 980ｓ,ｔｈｉｃｋｌａｙｅｒｏｆｉｔｓｂｌｏｏｍｃｏｖｅｒｅｄｔｈｅｌａｋｅｓｕｒｆａｃｅｅｖｅｒｙｓｕｍｍｅｒ,ａｔｔｒａｃｔ ｉｎｇｆｏ…     

SESTON AND DISSOLVED ORGANIC CARBON DYNAMICS DURING MICROCYSTIS BLOOM WITH SPECIAL REFERENCE TO THE EFFECTS OF THREE CHINESE DOMESTIC FISHES IN EXPERIMENTAL ENCLOSURES

ImODUonONGrowingconcemovertheprobboofobnoxiouswaterbloornspromPtedagreatdealofresearchintothebiologyofblue-grmalgae(Reynolds&Walsby,l975,Reynothe,l984).PreviousstudiesonMimptiSwereaimedatitSphySinlogicalcharaCteristirsindudinggroWthandbuoyancyraegnolds,l975),0Vendteringandphotosynthesisfea-turesffokamuraetal.,l984,Takamura,l985),nutrientdemandsoprlofTetal.,l952,0kado&Sudo,l979).TherearerepoffethatMicropstiswasawidelydistributalspedesthatcouldeasilybloomWhenambientenvironrnentSweresuit…     

Effect of enclosure and nutrient enrichment on Microcystis blooms in Donghu Lake

1 INTRODUCTION Surface blooms of nuisance cyanobacteria are often a signal of eutrophication (Jacoby et al., 2000). They are generally favored by conditions of low nitrogen to phosphorus ratios, high total phosphorus content, high water temperature, high …     

Numerical simulation of nutrient and phytoplankton dynamics in Guangxi coastal bays,China

The increasing riverine pollutants have resulted in nutrient enrichment and deterioration of water quality in the coastal water of Guangxi Province, China. However, the quantitative relationship between nutrient loads and water quality responses, which is crucial for developing eutrophication control strategies, is not well studied. In this study, the riverine fluxes of nutrients were quantified and integrated with nutrient cycling and phytoplankton dynamics by using box models for Guangxi coastal bays. The model concepts and biogeochemical equations were the same; while most model parameters were specific for each bay. The parameters were calibrated with seasonal observations during 2006–2007, and validated with yearly averaged measurements in 2009. The general features of nutrient and phytoplankton dynamics were reproduced, and the models were proved feasible under a wide range of bay conditions. Dissolved inorganic nitrogen was depleted during the spring algal bloom in Zhenzhu Bay and Fangcheng Bay with relatively less nutrient inputs. Phosphorus concentration was high in spring, which decreased then due to continuous phytoplankton consumption. Chlorophyll-a concentration reached its annual maximum in summer, but was the minimum in winter. Eutrophication was characterized by both an increase in nutrient concentrations and phytoplankton biomass in Lianzhou Bay. Either about 80% reduction of nitrogen or 70% reduction of phosphorus was required to control the algal bloom in Lianzhou Bay. Defects of the models were discussed and suggestions to the environmental protection of Guangxi coastal bays were proposed.     

EXPERIMENTAL STUDIES ON THE ROLE OF PLANKTIVOROUS FISHES IN THE ELIMINATION OF MICROCYSTIS BLOOM FROM DONGHU LAKE USING ENCLOSURE METHOD

The hypertrophic subtropic Donghu Lake's dense water bloom(of mainly Microcystis Anaabaena andOscillatoria)that occurred annually from the beginning of the 1970s, has disappeared since 1985. The infiuenceof planktivorous fishes (silver and bighead carps) on the water bloom was studied for three years usingthe enclosure method. The enclosures stocked densely with bighead and/or silver carp were free of waterbloom during the experimental period. The water bloom that appeared in the fish-free enclosures was completelyeliminated in 10-20 days by introduction of silver and/or bighead carp(grass carp was not effectivein controlling water bloom).This study showed clearly that grazing pressure by planktivorous fishes is a keyfactor in eliminating water bloom from the lake.     

ENCLOSURE EXPERIMENTS ON AND LACUSTRINE PRACTICE FOR ELIMINATING MICROCYSTIS BLOOM

Microcystis bloom, one of the most objectionable characteristics of eutrophication in tropical and subtropical waters, occurred in Donghu Lake (East lake) of Wuhan every summer from the 1970s up to 1984, but from 1985 up to now failed to occur there. The cause of its disappearance rema-ined in obscurity until recently. In situ enclosure experiments in the lake for three years showed that the stocking of the filter-feeding silver carp ( Hypophthalmichthys molitrix ) and big-head carp (Aristichthys nobilis ) played a decisive role in eliminating Microcystis bloom from the lake; but that recurrence of the bloom is possible under certain conditions. This paper presents the details and the results of enclosure ex-periments. The authors‘ analysis of fish biomass data obtained by echo-sounding and the fishery produc-tion of the lake over the years, revealed that the recurrence of Microcystis bloom can be prevented so long as the combined biomnss of silver carp and big-head carp remains at or exceeds 50 g per cubic meter of lakewater, as was the case in the lake‘s 1985 fish yield of 1015 t.     

Relative abundance of biotic communities of plankton and zoobenthos in three ox-bow lakes of Eastern India

We made comparative studies of three tropical ox-bow lakes in India with different trophic states. The species number of zooplanktons was negatively correlated with the degree of water eutrophication, and lakes of similar trophic status had higher similarity of species. The most significant differences of species composition were observed with rotifers and zoobenthos. The density and biomass of phytoplankton, rotifers, oligochaetes and chironomids tended to increase with increasing nutrients. We observed a significant difference of dominant species in different waters, especially the eutrophic lake, which was dominated by a few species of oligochaetes. The abundance of Tanypus bilobatus showed a highly significant correlation with the degree of eutrophication. Rotifers and zoobenthos showed considerably lower biodiversity in the eutrophic lake.     

Experimental studies on the role of planktivorous fishes in the elimination ofMicrocystis bloom from Donghu Lake using enclosure method

The hypertrophic subtropic Donghu Lake's dense water bloom (of mainlyMicrocystis, Anabaena andOscillatoria) that occurred annually from the beginning of the 1970s, has disappeared since 1985. The influence of planktivorous fishes (silver and bighead carps) on the water bloom was studied for three years using the enclosure method. The enclosures stocked densely with bighead and/or silver carp were free of water bloom during the experimental period. The water bloom that appeared in the fish-free enclosures was completely eliminated in 10–20 days by introduction of silver and/or bighead carp(grass carp was not effective in controlling water bloom). This study showed clearly that grazing pressure by planktivorous fishes is a key factor in eliminating water bloom from the lake. This work was supported by FEBL (State Key Laboratory for Freshwater Ecology and Biotechnology of China) funds and by the National Natural Science Foundation of China (Grant No., 3937014).     

The decline process and major pathways of Microcystis bloom in Taihu Lake, China

Eutrophication has become a serious concern in many lakes, resulting in cyanobacterial blooms. However, the mechanism and pathways of cyanobacteria decline are less understood. To identify and define the growth and decline of Microcystis blooms in Taihu Lake of China, and to illuminate the destination of surface floating blooms, we investigated the biomass distribution and variations in colony size, morphology, and floating velocity from October 2008 to September 2009. The results showed that the Microcystis bloom declined in response to biomass decrease, colony disaggregation, buoyancy reduction, and increased phytoplankton biodiversity, and these indicative parameters could be applied for recognition of the development phases of the bloom. Three major decline pathways were proposed to describe the bloom decline process, colony disaggregation (Pathway Ⅰ), colony settlement (Pathway Ⅱ), and cell lysis in colonies (Pathway Ⅲ). We proposed a strategy to define the occurrence and decline of Microcystis blooms, to evaluate the survival state under different stress conditions, and to indicate the efficiency of controlling countermeasures against algal blooms.     

Spatially different nutrient histories recorded by multiple cores and implications for management in Taihu Lake, eastern China

The Taihu Lake,a large shallow lake in the floodplain of the Changjiang(Yangtze) River in the eastern China,is faced with challenging ecological problems resulting from eutrophication,which has affected the regional freshwater supply of a large population.Although efforts have been made to assess the nutrient evolution histories in the northern bays,little is known regarding nutrient histories in different parts across the entire lake basin.In this paper,we present nutrient histories for different parts of the lake based on chironomid transfer functions applied to four short cores obtained from the northern,western and eastern regions of the lake.The chironomid-inferred total phosphorus(CI-TP) concentrations were compared with the phosphorus concentrations obtained by using instrumental and sedimentary data.The results suggest that trophic evolution histories were asynchronous throughout the lake during the past decades in response to different ecological regimes controlled by the nutrient input,wind direction and shoreline topography.The restoration of aquatic plants may be an effective option for the management of lake rehabilitation to ′natural′ conditions.Given the multiple factors controlling the biotic communities in such a large and complex lake,combined analyses among the multi-proxies encountered in the sediments are necessary for comprehensive insight into paleolimnological studies.The spatial heterogeneity in the ecological trajectories within this complicated ecosystem suggests that different management practices should be undertaken for specific lake zones in the Taihu Lake.     

近百年来龙感湖地区湖泊营养化过程

由于近年来社会经济的迅速发展，湖泊富营养化问题日趋严重。其中湿地的破坏是导致人湖营养盐增加的一个重要原因。对湿地变化与湖泊营养盐状况关系的分析是制定湖泊环境整治和生态修复的重要科学依据。湖泊沉积物含有丰富的生物和理化方面的信息，在缺乏长期湖泊监测记录的情况下。可以用来重建湖泊及其流域过去变化的历史。根据龙感湖表层沉积物^210Pb活度比变化，分析了该地区近百年来沉积物中湿地花粉、总磷和磁性参数．探讨了湖泊营养化过程及机理。研究表明，龙感湖近百年来营养级的增加是与湿生植被的破坏密切相关。20世纪上半叶的湖泊富营养化响应于磁性参数指示的流域土壤侵蚀速率的增加，而20世纪70年代以来湖泊营养程度的加重则与龙感湖流域农业化肥的使用和湿地植被破坏而导致湿地功能减弱有关。龙感湖流域内人类对湖周潍地的改造．破坏了湿地植被，助长了人湖物质的增加。湖泊营养相对富集，最终导致水体富营养化发生。     

基于MODIS数据的太湖浮游植物物候变化及其对水表温度的响应

浮游植物物候能够反映浮游植物的生长变化与湖泊生态系统的变化,水温、营养盐浓度等因素对物候有重要影响。太湖富营养化程度较高,水温的影响作用日趋显著,物候与水温关系的研究对理解、控制和改善太湖生态系统具有重要意义。本研究利用2003—2018年MODIS遥感数据计算浮游植物物候指标和湖泊水表温度（Temperature of Water Surface,LSWT）,通过分析太湖浮游植物物候时空变化特点探究了不同区域的物候特征,并结合LSWT揭示了浮游植物物候对LSWT变化的响应关系。结果表明：① 不同浮游植物物候指标具有不同空间分布特点,水华发生次数、峰值叶绿素a（Chla）浓度和水华总持续时间呈现由西部沿岸向湖心区递减的趋势;浮游植物生长开始时间和峰值Chla发生时间分布复杂但在沿岸区域相对较早;② 太湖可被划分为4种具有不同物候特征的区域,Ⅰ类区域主要位于贡湖湾、东部沿岸以及太湖中部开阔水域,该区Chla浓度范围为50~60 μg/L,且波动平缓,水华发生次数最少、开始最晚、持续时间最短;Ⅱ类区域主要分布于太湖西部沿岸,Chla浓度范围为50~90 μg/L且变化剧烈,该区水华发生次数最多、开始最早、持续时间最长;Ⅲ和Ⅳ类属于过渡区域,前者主要分布于梅梁湾、竺山湾及入湾口,后者主要位于南部沿岸以及太湖中部;③ 浮游植物物候对LSWT变化的响应受营养水平影响,当营养水平较高时,浮游植物的生长受LSWT的促进作用显著,LSWT年际变化的升高趋势对浮游植生长物候提前、生物量增加的影响明显,反之,则LSWT变化对浮游植物生长的影响减弱。     

Seasonal change of ice algal and phytoplankton assemblages in the Nella Fjord near Zhongshan Station, East Antarctica

The ice algal and phytoplankton assemblages were studied from Nella Fjord near Zhongshan Station, East Antarctica from April 12 to December 30, 1992. Algal blooms occurred about 3 cm thick on the bottom of sea ice in late April and mid November to early December respectively, and a phytoplankton bloom appeared in the underlying surface water in mid December following the spring ice algal bloom. The biomass in ice bottom was 1 to 3 orders of magnitude higher than that of surface water. Amphiprora kjellmanii, Berkeleya sp., Navicula glaciei, Nitzschia barkelyi, N. cylindrus /N. curta, N. lecointei and Nitzschia sp. were common in the sea ice temporarily or throughout the study period. The biomass in a certain ice segment was decreased gradually and the dominant species were usually succeeded as the season went on. Nitzschia sublineata and Dactyliosolen antarctica were two seasonal dominant species only observed in underlying water column. The assemblages between bottom of ice and underlying surface water were different except when spring ice algae bloomed. The evidence shows that the ice algal blooms occurred mainly by in situ growth of ice algae, and the phytoplankton bloom was mostly caused by the release of ice algae.     

Response of Cultural Lake Eutrophication to Hemp-retting in Quidenham Mere of England in Post-Medieval

To study the influence of human activity on natural lake, chironomid fauna change in the 700–560cm lake sediment in Quidenham Mere of England associated with chironomid inferred TP presented that the lake experienced a whole eutrophication process due to human hemp-retting in Post-Medieval based on the history record and pollen analysis, which was confirmed by mollusc and ostracod analysis. However, the response of chironomid and mollusc to retting was the strongest with ostracod a little behind. It proved that cultural eutrophication existed in history and could be recovered by itself despite of some long-term unachievable destroy. And it was most important for external nutrient to be cut off during lake restoration even in ancient times.     

基于贝叶斯方法的湖泊富营养化评价

对湖泊富营养化状态的有效评价是对湖泊进行有效管理的重要手段之一。针对富营养化评价中的不确定性,拟采用概率方法——贝叶斯方法对富营养化状态进行评价。以水利部水利水电规划设计总院提出的《地表水富营养化控制标准》为评价标准,选取Chla、TP、TN、CODMn、SD5个指标,对我国19个湖泊应用贝叶斯方法进行富营养化评价,并应用两个实例对该方法在富营养化评价中的有效性进行了验证。结果表明,贝叶斯方法应用于湖泊富营养化评价具有简单、实用的特点。     

ANALYSIS OF BIOLOGICAL GEOCHEMISTRY OFCHEMICAL ELEMENTS IN Betula ermanii FORESTIN CHANGBAI MOUNTAINS,CHINA

匠he 乙hangbal Mountains Nature Reserve Is a al，1982ZHUANGetal，1989；HUANGetal，1982；state－grade nature rese。e，also Is a component part of LIU，1993；LIU，1988； ZHU et al，1991）．Most ofreserve net of International blosphere．its area Is large，their research works focus on the chemical structure andthe natural environment and ecosystem lsprotected material cycling In alpine tundra zone andcompletely．the natural elements and ecological envl－broadleatKorean pine fores…     

Molecular characterization of cyanobacterial diversity in Lake Gregory, Sri Lanka

Eutrophication or the process of nutrient enrichment of stagnant waters due to excessive use of fertilizer is becoming a critical issue worldwide. Lake Gregory, an artificial lake situated in Nuwara Eliya, Sri Lanka was once a very attractive landscape feature and recreational area attracting a large number of visitors. Rapid urbanization in surrounding areas and the consequent intensification of agricultural and industrial activities led to eutrophication and siltation in the lake. Present study was conducted to detect cyanobacterial diversity and their ability to produce hepatotoxic microcystins using polymerase chain reaction (PCR)-based techniques. Twenty five water samples (surface and bottom) were collected from the lake and total nitrogen and total carbon were estimated. Cyanobacterial cultures were grown in appropriate media and microscopic observations were used to determine the morphological diversity of cyanobacteria isolated from different sites. Genomic DNA was isolated and purified from cyanobacteria using Boom’s method. DNA samples were analyzed by PCR with oligonucleotide primers for 16S rRNA gene and mcyA gene of the operon that encodes a microcystin synthetase. The 16S rRNA gene sequences revealed the presences of cyanobacteria belong to Synechococcus sp., Microcystis aeruginosa, Calothrix sp., Leptolyngbya sp., Limnothrix sp., order Oscillatoriales and order Chroococcales. The sequences obtained from this study were deposited in the database under the accession numbers (GenBank: GU368104-GU368116). PCR amplification of mcyA primers indicated the potential for toxin formation of isolated M. aeruginosa from Lake Gregory. This preliminary study shows that the Lake Gregory is under the potential risk of cyanobacterial toxicity. Clearly more work is needed to extend this finding and clarify if other cyanobacterial isolates have genetic potential to produce microcystin since this lake is utilized for recreational activities.     

Causes and control countermeasures of eutrophication in Chaohu lake,China

1 IN T R O D U C T IO NC haohu Lake,located in the centralA nhuiProvince ofC hina, has m ultiple functions of flood control, w atersupply,irrigation,transportation,fishery and tourism .Itis fam ous for its beautifullandscape and historic sites.For thousan…     

Recent insights into physiological responses to nutrients by the cylindrospermopsin producing cyanobacterium,Cylindrospermopsis raciborskii

The harmful cyanobacterium Cylindrospermopsis raciborskii is a widespread species increasingly being recorded in freshwater systems around the world. Studies have demonstrated some key attributes of this species which may explain its global dominance. It has a high level of flexibility with respect to light and nutrients, being capable of growth under low and variable light conditions. However, it is the strategy with respect to nutrient utilization that has received more attention. Unlike many bloom forming species, the dominance of this species is not simply linked to higher nutrient loads. In fact it appears that it is more competitive when phosphorus and nitrogen availability is low and/or variable. An important component of this flexibility appears to be the result of within-population strain variability in responses to nutrients, as well as key physiological adaptations. Strain variability also appears to have an effect on the population-level cell quota of toxins, specifically cylindrospermopsins(CYNs). Field studies in Australia showed that populations had the highest proportion of toxic strains when dissolved inorganic phosphorus was added, resulting in stoichiometrically balanced nitrogen and phosphorus within the cells. These strategies are part of an arsenal of responses to environmental conditions, making it a challenging species to manage. However, our ability to improve bloom prediction will rely on a more detailed understanding of the complex physiology and ecology of this species.     

Characteristics of dissolved organic matter in lakes with different eutrophic levels in southeastern Hubei Province,China

Dissolved organic matter(DOM) plays a crucial role in both the carbon cycle and geochemical cycles of other nutrient elements,which is of importance to the management and protection of the aquatic environme nts.To ac hieve a more comprehensive understanding the characteristic s of DOM in the Changjiang(Yangtze) River basin,water samples from four natural lakes(Xiandao,Baoan,Daye,and Qingshan) in southeastern Hubei Province in China with different eutrophication levels were collected and analyzed.The optical characteristics were analyzed using ultraviolet-visible spectrophotometry and excitationemission matrix spectroscopy coupled with parallel factor analysis.The results show that:(1) two humiclike components(C1 and C2) and two protein-like substances(C3 and C4) of DOM were identified in all waterbodies;(2) C3 originated primarily from the degradation of microalgae and contributed substantially to humic-like components during transformation.C4 was widely present in the Changjiang River basin and its formation was related to microbial activity,rather than algal blooms or seasons.Influenced by the water mixing,the protein-like components were more likely to be transformed by microorganism,whereas humiclike components were more easily to be photobleached;(3) the concentration of DOM and the fluorescence intensity of humic-like components gradually increased with rising lake eutrophication levels.With respect to protein-like components,only C3 showed changes along the eutrophication gradients;(4) DOM showed a high affinity with permanganate index(COD_(Mn)) and chlorophyll a(chl a) while the relationship was variable with phosphorus.This study helps us systematically understand the DOM characteristics,microbial activities,and pollutant transfo rmation in the Changjiang River basin and provide s reference to the ecological re storation of aquatic environments.