热带西太平洋Y3和M2海山微食物网主要类群生态分布与比较

2014年12月和2016年3月分别对热带西太平洋Y3海山(中层海山)和M2海山(浅海山)微食物网主要类群(包括聚球藻、原绿球藻、微微型真核浮游生物、异养细菌和浮游纤毛虫)丰度和生物量垂直分布进行了研究。结果表明,Y3和M2海山水文环境比较相似但略有区别,叶绿素最大值层(DCM)分别在75—100m和110m水层,微食物网各主要类群在垂直尺度上的分布与叶绿素a浓度紧密相关。其中浮游纤毛虫呈现"双峰型"模式,即丰度高值出现在表层和DCM层;原绿球藻和微微型真核浮游生物呈现"单峰型"模式,丰度高值出现在DCM层;聚球藻和异养细菌峰型相对不显著,DCM层以浅丰度较高,DCM层以深丰度明显降低。分析其原因,可能是受到温度、光照和营养盐的共同影响。Y3和M2海山微食物网结构的垂直变化不完全一致。其中,Y3海山30m以浅和150m以深异养细菌生物量占绝对优势,75—100m水层自养型生物(原绿球藻和微微型真核浮游生物)占绝对优势;M2海山75m以浅和200m以深异养细菌占绝对优势,110—150m自养型生物占绝对优势。M2海山自养型生物占优势的水层要明显深于Y3海山,可能与它们的海山类型和采样季节不同有关。     

热带西太平洋M4海山超微型浮游生物的生态分布特点

获取并分析了2017年8月热带西太平洋M4海山水体中的超微型浮游生物样品,根据流式细胞术的散射光和荧光信号,检测到M4海山各水层中普遍存在四个超微型自养浮游生物类群(聚球藻、原绿球藻、微微型真核浮游生物、微型真核浮游生物)和两个超微型异养原核生物类群(低核酸含量和高核酸含量异养原核生物)。聚球藻丰度高值出现在100m以浅;原绿球藻和微微型真核浮游生物丰度高值区在深层叶绿素最大值附近(75—150m);微型真核浮游生物和异养原核生物分布范围较广,150m以浅丰度较高。异养原核生物的生物量(1.68—11.25μgC/L)高于自养浮游生物(0.05—6.02μgC/L)的生物量。在超微型自养浮游生物中,原绿球藻生物量在100—150m水层占优势(53.83%±6.32%),微型真核浮游生物的生物量在75m以浅(58.62%±8.53%)和200—300m水层占优势(46.18%±7.82%)。在异养原核生物中,高核酸含量异养原核生物的生物量所占百分比(61.05%±3.98%)高于低核酸含量异养原核生物(38.95%±3.98%),然而在海山附近DCM层低核酸含量异养原核生物比例最高可达58.64%。冗余分析表明,超微型浮游生物的丰度与温度呈正相关,与深度和营养盐呈负相关关系。在M4海山超微型自养浮游生物分布没有明显的"海山效应",但海山的存在会对异养原核生物两个类群生物量的比例产生影响。     

南海东北部分粒级叶绿素a和超微型光合生物的周日变化

分别于2014年春、秋和2015年夏3个季节对南海东北部A站位(118°E,21.5°N)分粒级叶绿素a浓度和超微型光合生物(原绿球藻、聚球藻和超微型真核藻类)细胞丰度的昼夜变化进行了24 h时间序列连续观测和分析。通过萃取荧光法分析叶绿素a浓度,发现叶绿素a浓度呈现明显的昼夜变化,春季正午最高,秋季和夏季基本变化趋势为白天升高,夜晚降低;而因夏季中午的光抑制作用,叶绿素a的浓度相对较低。超微型光合生物(0.2~3μm)对总叶绿素a的贡献最高(71.49%),小型浮游植物(20μm)贡献率最低(10.41%)。通过流式细胞技术检测到3个超微型光合生物类群;其中,原绿球藻为优势类群,最大细胞丰度达1.05×10~5cells/m L,其次是聚球藻,超微型真核藻类的细胞丰度最低,但由于其单位细胞内的叶绿素a含量高,所以可能对叶绿素a的贡献最大。聚球藻丰度基本上白天下降,傍晚到午夜上升;秋季和夏季,超微型真核藻类的丰度白天高,夜晚低,而春季则相反;原绿球藻在秋季和春季的昼夜变化规律和超微型真核藻类相似。在多种因素共同影响下,光照是调控叶绿素a浓度和超微型光合生物丰度昼夜变化的一个关键因素。季节变化上,原绿球藻的细胞丰度季节间没有统计学差异(P0.05),聚球藻的季节变化为秋夏春,超微型真核藻类的季节变化规律和聚球藻相反。     

2009年冬、夏季南海北部超微型浮游生物的分布特征及其环境相关分析

2009年2月(冬季)和8月(夏季)在南海北部海域(nSCS)采用流式细胞术对聚球藻、原绿球藻、超微型光合真核生物3类超微型光合浮游生物和异养浮游细菌的丰度和碳生物量的时空分布特征进行了研究,并分析了其与环境因子之间的关系。结果表明,夏季聚球藻和原绿球藻的平均丰度高于冬季,超微型光合真核生物和异养浮游细菌的丰度反之,为冬季高于夏季。聚球藻、超微型光合真核生物和异养浮游细菌在富营养的近岸陆架海域丰度较高,而原绿球藻高丰度则出现在陆坡开阔海域。在垂直分布上,聚球藻主要分布在跃层以上,跃层以下丰度迅速降低;原绿球藻高丰度主要出现在真光层底部;超微型光合真核生物在水层中的高值同样出现在真光层底部,且与Pico级份叶绿素a浓度分布一致;异养浮游细菌在水体中的分布与聚球藻类似。这些分布格局的差异,取决于环境条件的变化和4类超微型浮游生物生态生理适应性的差异。在超微型光合浮游生物群落中,各类群碳生物量的贡献因季节和海域类型的不同而发生变化:聚球藻在夏季近岸陆架区占超微型光合浮游生物总碳生物量的41%,原绿球藻在陆坡开阔海成为主要贡献者(50%),超微型光合真核生物碳生物量以冬季为高(在近岸陆架区占比68%)。冬、夏季异养浮游细菌碳生物量均高于超微型光合浮游生物碳生物量。     

夏末亚热带太平洋至楚科奇海微微型浮游植物群落

依托2019年8月至2019年9月中国第十次北极科学考察,基于流式细胞术,对亚热带太平洋至楚科奇海的表层微微型浮游植物丰度、细胞大小和色素含量的变化特征进行了分析,研究了各类群的环境适应性。结果表明,微微型浮游植物群落未检出原绿球藻,仅包含聚球藻和微微型真核藻,其丰度范围分别为(0.1～46.7)×10~3个/mL和(1.1～43.2)×10~3个/mL;聚球藻和微微型真核藻丰度高值均出现在白令海陆架区,其丰度升高与营养盐浓度升高有关;聚球藻最小丰度出现在楚科奇海,而微微型真核藻最小丰度出现在亚热带太平洋。聚球藻和微微型真核藻的侧向散射光(与细胞大小有关)和色素荧光强度均存在两倍以上的变化,与纬度或温度不存在显著的相关关系。在相同温度条件下,营养盐限制会降低微微型浮游植物的细胞大小。聚球藻的细胞大小与色素含量不相关,而在微微型真核藻中两者存在同向变化。在全球气候变暖和北极冰盖减少背景下,微微型浮游植物在北极生态系统中的作用会增加,对食物网和生态系统功能产生重要影响。     

微微型浮游植物的生态学研究进展

自1979年聚球藻(Synechococcus)被发现以来,对以前为人们知之甚少的微微型(粒径<2μm)、超微型(粒径<5μm)浮游植物的研究逐渐形成并日益发展起来,并成为当前海洋生态学研究的热点之一。到目前为止,这类广布于各种水体中的微小生物可分为聚球藻、原绿球藻(Prochlorococcus)和微微型真核浮游植物(eukaryotic picophyPlankton)3大类。     

南大洋和南极考察航线海表超微型浮游生物分布及影响因子

南极考察航线跨越全球多个大洋,对认识超微型浮游生物在全球尺度分布及变化具有重要意义。依托中国第33次南极考察,利用流式细胞仪对航线海表水样中的超微型浮游生物进行了现场测定。结果表明,在热带与温带海域,超微型浮游生物量可占总叶绿素a浓度的60%以上,在南大洋则集中在15%～40%;原绿球藻主要分布在40°S以北海域,聚球藻主要分布在50°S以北海域,而超微型真核藻类和异养细菌则在沿线各海域均有明显分布。在纬向上,原绿球藻、聚球藻、超微型真核藻类和异养细菌的平均丰度分别为(5.50±9.09)×10³ cells/mL、(13.56±20.33)×10³ cells/mL、(3.87±3.08)×10³ cells/mL和(6.39±4.78)×10⁵ cells/mL;南大洋海域,超微型真核藻类和异养细菌的平均丰度分别为(3.31±1.46)×10³ cells/mL和(4.68±4.39)×10⁵ cells/mL,在少数站位检测到较低丰度的聚球藻,平均值...     

辽东湾微微型浮游生物的丰度及季节变化

本研究自2006年7月至2007年10月,分4个季节调查了辽东湾海域的微微型浮游生物的丰度。结果表明:夏季辽东湾的聚球藻(Synechococcus)丰度最高,平均丰度为1.89×104 cell/mL。秋季次之,冬季居第三位,春季最低。夏季比其他3个季节的聚球藻丰度要高一个数量级。夏季辽东湾的微微型真核藻类(Picoeukaryote)丰度最高,平均丰度为3.79×103cell/mL,秋季次之,冬季与春季相差不大。夏季比其他3个季节的微微型真核藻类丰度要高一个数量级。     

北部湾海域微型、微微型浮游植物类群季节变化及其与棕囊藻赤潮的关系初探

近年来，北部湾海域连年暴发球形棕囊藻（Phaeocystis globosa）赤潮，对近海生态环境构成了潜在威胁。为了解北部湾海域球形棕囊藻赤潮生消过程及其与其他浮游植物类群的关系，自2016年9月至2017年8月期间，在北部湾海域开展了9个航次的调查，并应用流式细胞仪分析了微型真核藻类、微微型真核藻类、原绿球藻（Prochlorococcus spp.）和聚球藻（Synechococcus spp.）等4类浮游植物类群的变化情况。结果表明，北部湾海域各浮游植物类群主要分布在调查区北部钦州湾外侧海域和雷州半岛西侧近岸海域，其丰度有明显的季节变化。微微型真核藻类在春、秋两季丰度较高，而冬、夏季较低。微型真核藻类、聚球藻和原绿球藻的丰度均呈现夏季高、冬季低的特点，其中聚球藻丰度的季节变化最为显著，与水温变化密切相关。结合对球形棕囊藻赤潮生消过程以及调查海域水文条件的分析可以看出，北部湾海域球形棕囊藻赤潮发生过程中，海水温度下降至20℃左右，并且聚球藻和原绿球藻丰度具有明显变化，有望作为棕囊藻赤潮的预警指标，为该海域棕囊藻赤潮的预测和防控提供依据。     

基于光合色素化学分类和流式细胞术结合的珠江口自养微微型浮游生物种群时空分布特征

为探究珠江口海域自养微微型浮游生物种群时空分布特征及其与环境之间的关系,于2013年5～11月,运用高液相色谱(HPLC)法和流式细胞术对珠江口海域表层水体中微微型浮游生物进行测定。流式细胞计数结果显示,珠江口海域自养微微型浮游生物由聚球藻(Synechococcus, Syn)和微微型真核生物(Picoeukaryotes,PEUK)组成。聚球藻始终占据总细胞丰度的主导地位。光合色素化学分类法(Chemotaxonomy,CHEMTAX)分析表明,自养微微型浮游生物群落结构具有明显的季节性变化,春季和夏季生物量以聚球藻为主,秋季生物量以青绿藻为主。CHEMTAX分析和流式细胞计数结果的相关性分析表明,在春季和夏季Syn细胞丰度与CHEMTAX生物量(即Syn贡献chla)之间呈现极显著正相关(P<0.01),PEUK细胞丰度与CHEMTAX生物量(即PEUK贡献chla)也存在显著正相关(P<0.05);然而,在秋季则无显著性相关关系(P>0.05)。冗余分析表明,温度和营养盐浓度是影响自养微微型浮游生物群落分布与组成的重要因素。另外,盐度、透明度、悬浮颗粒物对自养...     

The beam attenuation to chlorophyll ratio: an optical index of phytoplankton physiology in the surface ocean?

The particulate beam attenuation coefficient (c_p) is proportional to the concentration of suspended particles in a size domain overlapping that of the phytoplankton assemblage. c_p is largely insensitive to changes in intracellular chlorophyll concentration, which varies with growth irradiance (a process termed ‘photoacclimation’). Earlier studies have shown that the ratio of c_p:chlorophyll (i.e., c_p^*) exhibits depth-dependent changes that are consistent with photoacclimation. Similar relationships may likewise be expected in the horizontal and temporal dimensions, reflecting changes in mixing depth, incident irradiance, and light attenuation. A link between c_p^* and more robust photoadaptive variables has never been explicitly tested in the field. Here we use five historical field data sets to directly compare spatial and temporal variability in c_p^* with two independent indices of photoacclimation: the light-saturated, chlorophyll-normalized photosynthetic rate, P^b_opt, and the light-saturation index, E_k. For the variety of oceanographic conditions considered, a first-order correlation emerged between c_p^* and P^b_opt or E_k. These simple empirical results suggest that a relationship exists between a bio-optical variable that can potentially be retrieved remotely (c_p^*) and physiological variables crucial for estimating primary productivity in the sea.     

Factors affecting clarity of New Zealand lakes

Average values of inherent optical properties for the 400–700 nm waveband were estimated from quantum irradiance measurements on 27 New Zealand lakes of diverse optical character, using published nomograms. Secchi disc depths, turbidity, algal pigment, non‐volatile suspended solids, and absorption by membrane‐filtered samples at 440 nm (g 440 ) were also measured. Turbidity (NTU) correlated closely with the scattering coefficient (m^‐1) and these quantities were almost numerically equal, as found in other studies. The data were found to conform to an expression in the oceanographic literature relating Secchi disc depth to the beam attenuation and diffuse attenuation coefficients. Specific beam attenuation, scattering, and absorption coefficients were estimated from the coefficients of linear multiple regressions of the measured total coefficients on the variables: total pigment (chlorophyll a + phaeopigment), non‐volatile suspended solids, and g 440. The estimated values were in reasonable agreement with similar specific coefficients reported in the oceanographic literature. The coefficients provide a basis for predicting clarity in new impoundments or for predicting the effects of loading changes (e.g., of nutrients) on the clarity of existing lakes. The coefficients can be used for classifying lake waters into different optical types.     

Meridional distribution and carbon biomass of autotrophic picoplankton in the Central North Pacific Ocean during late northern summer 1990

The meridional distribution of autotrophic picoplankton groups in the central north Pacific was studied during the late northern summer of 1990. Sampling was along a section at 175°N which extended from 45°N to 8°S. The section is far from coastal regions and included subarctic, central gyre, and equatorial areas. Five autotrophic picoplankton groups, autotrophic microflagellate, red-fluorescing picoplankton,Synechococcus, prochlorophyte, and orange-fluorescing picoplankton, were identified from samples taken at stations distributed along this section. These five groups showed distinctive differences in their meridional and vertical distributions. The autotrophic microflagellates and red-fluorescing picoplankton showed distributions that were similar to that of chlorophyll a, which was dominated by the <3 μm size fraction. However, the vertical distribution of these groups was different.Synechococcus was found mostly in surface waters (PAR<10%) and was particularly abundant in the Kuroshio Extension and south of the equatorial region where the nitracline was shallow (50–75 m). Prochlorophytes were abundant in the deep euphotic layer (PAR 1-0.1%) from the south of the Kuroshio Extension to the south of the equatorial area. Orange-fluorescing picoplankton, which may be one kind of cyanobacteria but is larger than typical Synechococcus, were mostly distributed in the oligotrophic surface waters of the central gyre. The carbon biomass estimates for these organisms showed that these five groups dominated in different areas. The vertical distribution of carbon biomass did not correspond to that of chlorophyll a in the central gyre and south of the equator because of the larger carbon/ chlorophyll a ratio of Synechococcus and orange-fluorescing picoplankton relative to that of the other picoplankton.     

红树林植株形态及种植密度对消波能力影响的数值模拟研究

本研究利用非结构网格有限体积海洋模型FVCOM(Finite-Volume Coastal Ocean Model)、MDO(Mellor-Donelan-Oey)波浪模型以及可分层的植被波耗散参数化方案,通过对比有无红树林工况评估红树林的波浪衰减能力,研究了不同形态的红树林在高水位与低水位情况下的消波能力,分析了植株密度对红树林消波能力的影响。结果表明,红树林的消波系数与林带宽度呈非线性正相关,与水深的关系则与红树结构有关。以白骨壤(Avicennia marina)为代表的A型植株形态在低水位下的消波系数极大值为67.9%,高水位下则能够达到94.4%;以红海榄(Rhizophora stylosa)为代表的B型植株形态的消波系数在低水位和高水位的极大值分别为90.6%、89.4%;以角果木(Ceriops tagal)为代表的C型植株形态的消波能力在高低水位区别不大。植株密度的增加能提高红树林消波系数上限,还能使得红树林消波系数对林带宽度的变化更为敏感,减小达到消波系数极大值所需要的林带宽度。在保护现有的红树林生态系统或建立新的红树林综合减灾防护系统时,应注意不同植株结构红树林的...     

Correlations between the inherent hydrooptical characteristics in the spectral range close to 550 nm

Correlations of the single scattering albedo ω₀ and the probability of backscattering \(\tilde b_b \) with the beam attenuation coefficient c are presented for a wavelength of 550 nm in waters of cases 1 and 2. Correlations based on more than 70 measurements in different regions of the World Ocean in the range of c from 0.08 to 2.5 m^?1 are compared with the data of observations of c and ω₀ available in the literature.     

Tracing offshore low-salinity plumes in the Northeastern Gulf of Mexico during the summer season by use of multispectral remote-sensing data

To trace offshore surface low-salinity water (LSW) in the northeastern Gulf of Mexico, a proxy was developed using the surface water beam attenuation coefficient (c _p), and salinity matched with synchronous Sea-viewing Wide Field-of-view Sensor (SeaWiFS) satellite data from three annual summer cruises (July 1998–August 2000) using a two-step empirical approach. First, a relationship between in-situ salinity and c _p was obtained. Second, in-situ c _p was matched with SeaWiFS radiance ratios of all available blue-to-green wavelengths. Finally, satellite-derived surface salinity was determined directly by combining the two empirical relationships, providing a robust estimate over a range of salinities (26–36). This significantly improves the limited spatial and temporal resolution of surface salinity distribution obtained by shipboard sampling. The resulting correlation is best explained as mixing between low-salinity plume waters and normal salinity Gulf waters. The empirical relationships were used to map satellite-derived salinity using the average of SeaWiFS images during each summer cruise. As expected for summer, spatial patterns of LSW plumes with high c _p, particulate matter (PM), particulate organic carbon (POC), and chlorophyll-a (Chl-a) were connected to the mouth of the Mississippi River Delta and extended to the east-southeast. Normal salinity Gulf water with lower c _p, PM, POC, and Chl-a was confined to the shelf and upper slope in the eastern part of the study area, outside the plumes. This proxy approach can be applied throughout the region of shipboard sampling for more detailed coverage and analysis.     

Sediment–water interactions of natural oestrogens under estuarine conditions

The natural human female hormones oestrone and 17β-oestradiol have been implicated in the disruption of endocrine systems in some wildlife adjacent to sewage effluents. The sorption behaviour of these two compounds under estuarine conditions was studied by spiking either 2.55 μg of oestrone or 2.65 μg of 17β-oestradiol in kinetic experiments. In equilibrium experiments, 3 ng of oestrone or 3.2 ng of 17β-oestradiol was added in each of the centrifuge tubes. Sorption onto sediment particles was relatively slow, with sorption equilibrium being reached in about 70 and 170 h for oestrone and 17β-oestradiol, respectively. The effects of a variety of environmental parameters on sorption were studied including salinity, sediment concentration (SC), the presence of a third phase, particle size and, also, surfactant concentrations. Results show that although salinity did not induce any statistically significant effect on the sorption of 17β-oestradiol, it did statistically enhance the sorption of oestrone, and a salting constant of 0.3 l mol⁻¹ was derived. The partition coefficient for both compounds decreased with increasing sediment concentration, a phenomenon that has been widely reported and attributed to the presence of colloids (which could enhance dissolved concentrations). In this paper, the true partition coefficients for sediment particles (K_p^true) and colloidal particles (K_c^true) have been calculated, and a K_p^true value of 141 and 102 ml g⁻¹ was obtained for oestrone and 17β-oestradiol, respectively. In addition, K_c^true values for oestrone (222×10² ml g⁻¹) and 17β-oestradiol (135×10² ml g⁻¹) were two orders of magnitude higher than their respective K_p^true values, suggesting that the colloidal particles are significantly stronger sorbents for natural oestrogens than sediment particles. Particles of different sizes were found to have different partition coefficients due to the strong relationships between partition coefficients for the two compounds and particulate organic carbon (POC) contents and specific surface areas (SSAs). The presence of a surfactant was shown to reduce the partition coefficients for the two compounds, although its concentrations being used were higher than those normally found in the natural environment.     

Investigation of Cd, Cu, Ni and Th in the colloidal size range in the Gulf of Maine

Size-fractionated seawater samples were collected from the Gulf of Maine to determine the fraction (f_c/d) of total dissolved (< 1 μm) Cd, Cu, Ni and ²³⁴Th in the colloidal size range (1,000 nominal molecular weight, NMW, to 1 μm) using cross-flow filtration. Colloidal Cd, Cu and Ni represents < 1–7% of the total dissolved concentration in these shelf waters and increases with an increase in particle concentration. By comparison, results obtained for particle-reactive ²³⁴Th indicate that < 1–47% of total dissolved is associated with the colloidal size fraction. A revised relationship between the concentration of colloids (C_c) and suspended particles (C_p) is reported (log C_c = 0.66 log C_p −2.01 kg L⁻¹) and used to examine the dependence of f_c/d for these metals on the concentration of suspended particles for C_p = 0.01–100 mg L⁻¹. Results indicate that a significant fraction (˜ 10–30%) of Cd, Cu, Ni and ²³⁴Th in the traditionally defined ‘dissolved’ fraction may exist in the colloidal size range in regions characterized by high particle concentrations (C_p > 1–10 mg L⁻¹), such as in near-shore and estuarine waters.     

Analysis of ocean color spectra (I)

By using the two-flow optical model, the variation in the irradiance reflectance of the ocean just below the surface has been calculated for varying water optical properties, for the interpretation of remotely-sensed ocean color data. The input variables used in our model are the concentration of phytoplankton (chlorophyll-a), the absorption coeffiicient of yellow substance, the particle scattering coefficient, the ratio of the back-scattering coefficient to the total scattering coefficient of particles, and the ratio of the absorption to scattering coefficients for particles.The irradiance reflectance increases monotonically with the ratio of the back-scattering coefficient to the total scattering coefficient. Spectral changes occur in the irradiance reflectance for increases in chlorophyll-a concentration and yellow substance as well as the ratio of the absorption to scattering coefficients.Because slightly different mathematical expressions have been derived by other investigators using the two-flow model, an evaluation of the resulting calculation differences is presented and discussed.