苏格兰阿伯丁城市土壤的微生物特性研究

在重金属的胁迫下,阿伯丁城市土壤中微生物特征发生了显著的变化,与农村土相比,微生物基底呼吸作用明显增强,但微生物生物量却显著降低,微生物生理参数Ｃｍｉｎ／Ｃｏｒｇ、ｑＣＯ２值明显升高,Ｂｉｏｌｏｇ数据显示城市土壤对能源碳的消耗量和速度显著升高,而且对能源碳的利用方式发生了改变,但利用效率却明显降低,反映出微生物特性可作为城市土壤环境质量变异的有效指标。     

用微生物对单一碳源利用方法探讨重金属在城市土壤中积累的环境效应

Biolog研究表明，英国阿伯丁市城市土壤的微生物群落结构显著有别于农村土壤，并使微生物对碳源的消耗量增加，消耗速度加快。城市土壤中不仅重金属Cu,Pb,Zn,Ni的含量明显高于农村土壤，而且其化学形态的主成分分析表明，有效态Pb,Zn,Cu及有机态Ni和Cu是导致城市土壤区分于农村土壤的主要因子。相关性分析表明，Biolog的这种变化规律与重金属的上述化学形态密切相关；典型变量分析表明，重金属对城市土壤微生物群落结果的这种损伤具有长期性效应及不可恢复性。     

城市热岛效应对土的工程性质影响及灾害效应分析

随着世界各国,尤其我国的城市化进程的不断加快,城市规模不断扩大,城市中高层建筑的密度越来越高,城市热岛效应（UHIE）及其带来的各类环境问题日益突出。对于城市热岛效应的研究目前主要集中在城市范围内的大气部分,对于它对城市赖于存在的地质环境的影响研究很少,而对土的工程性质的影响及其灾害效应常被忽视。从UHIE与城市地下土体工程性质间的关系出发,介绍了UHIE的成因和发展程度,剖析了城市环境中的土体与自然环境中土体的差别以及UHIE影响土体工程性质的方式,初步分析了UHIE对土的工程性质的影响及其引起的灾害效应。分析成果对于开展城市热岛效应环境中土的工程性质变化及其灾害效应研究有重要的指导意义。     

城区与郊区土体湿度监测与对比分析——以南京市为例

由城市化进程改变的城市空间热环境对城区土体湿度产生了重要影响。为了了解城区与郊区土体湿度的差异,以南京市为例,分别在城区、郊区建立了土体湿度监测站,分析2009年6月1日至2010年6月7日南京城区、郊区地下1 m范围内裸土、草地及混凝土覆盖环境下土体湿度的时空演化特征。研究结果表明,南京城区土体湿度总体上小于郊区,存在显著的城市土体"干岛效应",年平均干岛强度为-7.4%。在时间尺度上,1月的干岛强度最小,为-2.1%;7月最大,达到-20.5%。在空间尺度上,郊区土体湿度随深度增加而增大,城区土体则无明显规律。在不同地面覆盖环境下,城区、郊区土体湿度变化规律不同:城区裸土环境下土体湿度日变化明显,而草地及混凝土下的土体湿度日变化相对较小,3种地面覆盖环境的年均土体湿度变化规律为草地(19.0%)<混凝土(26.4%)<裸土(29.5%);郊区3种地面覆盖环境下土体湿度日变化区间及变化频率均比城区大,且年均土体湿度为混凝土(27.4%)<草地(34.7%)<裸土(36.2%)。最后,分析了造成城区、郊区土体湿度差异的原因。     

城市热岛效应对土体工程性质的影响及其关键科学问题

随着全球气候变暖、城市化进程加快,世界各地大城市的热岛效应及其带来的各种环境和生态问题越来越严重。本文从地质环境保护的角度,分析了城市热岛效应的成因、特点及其对地质环境的影响,重点讨论了城市热岛效应环境中土体工程性质的变化及其带来的各种灾害效应,在此基础上,凝练出了4个关键科学问题,即城市热岛效应环境中土体温度场变化规律、土体中水分迁移规律、土体工程性质变化规律以及土体地质灾害效应,并对它们的具体研究内容进行了详细的分析。论文的分析成果对于掌握城市热岛效应对土体工程性质的影响,保护城市地质环境和防灾减灾,实现城市的可持续发展具有重要的理论和现实意义。      

艾比湖地区冻融作用对土壤微生物数量和群落结构的影响

通过对艾比湖地区冻融阶段7个典型样地野外采样和室内分析,研究了冻融作用条件下土壤微生物数量与土壤性质的关系、土壤微生物数量变化特征和土壤微生物群落结构的变化特征. 结果表明：不同冻融阶段,不同种类微生物数量受到不同土壤理化因子的制约. 1月,细菌数量与土壤有机质含量呈显著正相关(P<0.05),真菌数量与土壤含水量和土壤有机质含量呈极显著正相关(P<0.01),与土壤全氮和土壤孔隙度呈显著正相关(P<0.05);3月,只有真菌数量与土壤含水量呈极显著正相关(P<0.01);4月,放线菌数量与土壤有机碳和全氮含量呈显著正相关(P<0.05),然而与土壤pH值呈显著负相关(P<0.05). 总体来讲,冻结期土壤微生物数量较低,冻融后期(4月)达到峰值,其中,7 a 撂荒地的微生物数量最高,3 a撂荒地最低. 在研究区寒冷季节,微生物类群中放线菌占主导地位(81.9%);融化阶段,则是细菌占主要地位(52.1%~53.9%).     

Soil bacterial functional diversity as influenced by cadmium,phenanthrene and degrade bacteria application

There are increasing concerns over compound pollution in northeast of China and its bioremediation. Cd, Phe, degradation bacteria W.J and their combination on soil microbial community function were assessed with the BIOLOG method. The microbial community was extracted from the soil with different treatments. Average color development, richness, Shannon–Weaver index, and principal component analysis on OD standardized data were performed. The results showed that soil microbial metabolic diversity in CK was significantly higher than that in the soils in which Cd, Phe, W.J or their combination was added. It indicated that Cd, Phe has significantly negative effect on the sole-carbon-source metabolic ability of soil microbial communities, particularly when both of them were present. Application of degradation bacteria incubation makes soil microbial communities same as control except for the significant decrease in 4-hydroxy benzoic acid, l-serine, glucose-1-phosphate metabolism. It shows that the incubate application has a considerably effect in soil communities reversion.     

基于SNTHERM雪热力模型的东北地区季节冻土温度模拟

冬季土壤温度在土壤肥力、植被安全越冬、土壤微生物活动中扮演着重要角色。雪盖的反照与隔热作用对冬季土壤温度变化及冻融过程具有一定影响,深入探究积雪覆盖对土壤温度的影响机制有十分重要的意义。雪热力模型（Snow Thermal Model,SNTHERM）是用来模拟和预测积雪演化和冻土温度的一维质能平衡模型。基于该模型,结合积雪下冻土温度的观测试验,通过模型模拟结果与实测数据的统计特征参数分析,进行了积雪覆盖下冻融土壤温度变化过程模拟的有效性和精度评价。结果表明,在积雪覆盖条件下,SNTHERM模型能够有效地模拟雪盖下浅层（5 cm、10 cm、15 cm深度）冻土日平均温度的变化过程,模拟值与观测值具有很好的一致性。通过改进模型中土壤层水分迁移等因素,能够提高冻土温度的模拟精度,为研究积雪各参数演化过程与下垫面温度的相互作用奠定理论基础,有助于提高积雪参量空间遥感的反演精度。     

Did Tsunami tremor jolt microbial biomass and their activities in soils? A case study in Andaman Island,India

The nature of selected soil-chemical and microbial properties influenced by tsunami affected and non-affected areas along the border areas of the alluvial Andaman Island in India were investigated. Soils of these areas have turned saline and saline-sodic due to the ingression of sea water. The electrical conductivity of the saturation extract of the surface soil varied from 11.2 to 23.8 dS m^?1 in 2005, and it was decreased to 0.8–10.3 dS m^?1 in 2006 due to the heavy rain in the following year after the tsunami. Soil quality indicators, like microbial biomass C, microbial metabolic quotient, microbial respiration quotient and fluorescein diacetate hydrolyzing activity, decreased in the tsunami affected soil in 2005, but slightly increased in 2006. All microbial parameters were significantly negatively correlated with the electrical conductivity, sodium absorption ratio and exchangeable sodium percentage. Suppression of microbial biomass and their activities in the soils due to the increased-salinity is of great agronomic significance and needs suitable intervention for sustainable crop production. Significant differences were found in soil-chemical and microbial characteristics between tsunami affected and non-affected areas. Hierarchical clustering algorithm on the basis of different soil-chemical and microbial characteristics revealed that there is significant difference in grouping between tsunami affected and non-affected zones. From this study, it can be concluded that the sea water ingression detrimentally influenced the microbial properties of tsunami affected soil.     

不同碳酸盐岩和土层厚度下土壤微生物数量及生物量的研究——以贵阳市花溪区为例

碳酸盐岩发育土壤的厚度变幅大,通过野外挖掘调查,在贵阳市花溪区分薄土、中土及厚土3种土层类型研究喀斯特地区不同土层厚度下微生物数量及生物量,结果表明:随着土层厚度的增加,土壤的细菌、真菌、放线菌及微生物总数逐渐增加;细菌在土壤微生物中占据了绝对优势,在石灰岩及白云岩发育土壤的各土层中分别占88.13%、85.71%、87.36%、85.00%及77.78%;石灰岩发育的土壤从薄土到厚土微生物量碳（C）、氮（N）、磷（P）的含量逐渐增加,分别增加15.15 mg·kg-1、2.82 mg·kg-1、0.18 mg·kg-1,白云岩从薄土到中土微生物量C、N、P的含量分别增加5.13 mg·kg-1、0.39 mg·kg-1、0.10 mg·kg-1;在同等厚度下石灰岩发育的土壤微生物量N、P含量明显低于白云岩发育的土壤,中土中差异最大,分别相差0.90 mg·kg-1和0.21 mg·kg-1,而石灰岩发育土壤的中土中微生物量C的含量则高于白云岩发育的土壤,高17.31 mg·kg-1;探讨相关性,pH值对微生物量C、P有显著影响（p<0.05）,全P含量对微生物量P有极显著影响（p<0.01）。      

冻融交替对不同年代排水造林湿地土壤微生物活性及有机碳密度的影响

以小兴安岭湿地土壤为研究对象,基于室内分析和冻融实验,分析了冻融作用下不同年代排水造林湿地土壤微生物量、酶活性以及有机碳密度的变化趋势,探讨了不同年代排水造林湿地土壤微生物活性与有机碳密度之间的相关关系,以期为深入认识冻融期间高寒高纬度地区土壤碳循环过程提供参考依据。结果表明：（1）冻融次数对土壤微生物量碳、氮含量影响显著（P<0.05）,经历9次冻融循环后,土壤微生物量碳、氮含量比冻融前明显减少;在三种不同年代排水造林的湿地中,排水时间越短,土壤微生物量碳、氮含量下降幅度越大,表明长时间的反复冻融交替可能导致土壤微生物量的进一步减少。（2）冻融前后,土壤蔗糖酶和淀粉酶活性均表现为下降趋势,且多次冻融交替后,-25~5℃冻融处理比-5~5℃冻融处理酶活性更低,表明较大的冻融温差更能降低土壤酶的活性度。（3）随着冻融次数和冻融温度的变化,四种湿地的土壤有机碳密度基本保持稳定,但其与土壤微生物量、酶活性却存在着高度的正相关性,通过探究微生物活性所调控的土壤过程,可以直接或间接了解土壤有机碳密度的变化趋势,便于从本质上验证其响应机制。     

岩溶土壤的生态地球化学特征及其指示意义——以贵州贞丰—关岭岩溶石山地区为例

岩溶石山地区土壤退化与恢复的成因和机理一直是近年来土壤学和生态学研究的热点。以贵州西南部贞丰—关岭岩溶石山区为对象，选择了区内退化地、农用地、恢复地等生态样块以及与未退化地为对照，采集了其中的表层和亚表层土壤，分别进行了土壤的养分库量、微生物活性与功能和土壤酶活性等化学分析。研究表明土壤养分库是土壤中微生物活性基础，微生物量碳随土壤养分库容的退化或恢复而相应变化，退化岩溶地植被恢复3~6年后，主要养分总库容得到明显恢复(恢复程度55%~65%)，因而带动了微生物量碳的恢复（平均恢复程度60%以上），但养分的活性（有效磷）、微生物的功能（呼吸熵及土壤脲酶和磷酸酶活性）并没有得到相应程度的恢复（平均恢复程度仅在25%~40%）。这些结果提示，限制性养分活性和微生物功能的恢复是植被恢复下生态系统健康水平的重要指示。因此，在分析岩溶土壤和生态系统退化过程的本质以及评价生态恢复的效应时，不仅应将微生物量碳和总养分库指标作为岩溶土壤退化恢复的指标，更应将微生物区系的质量和功能指标纳入关键评价内容。      

外源氮输入对草地土壤微生物特性影响的研究进展

大气氮沉降作为全球变化的重要现象之一,沉降量不断增加所带来的一系列生态问题日趋严重.草地作为陆地生态系统的主体类型之一,对大气氮沉降的响应不仅体现在地上植被生长和群落动态的变化,其地下各种生态过程的变化更加值得关注.此外,世界范围内的草地生态系统大部分面临不同程度的退化,草地施氮是寻求草地恢复的有益尝试.综述了大气氮沉降和人为施氮引起的外源氮输入变化对草地生态系统土壤微生物特性(微生物数量、微生物量、微生物呼吸、微生物多样性和土壤酶)影响的研究进展,研究表明:①施氮有利于细菌数量的增加,但对真菌数量的影响甚微或是降低真菌的数量.②长期施氮降低土壤微生物量,但短期施氮的影响效应具有不确定性.③施氮对草地土壤微生物呼吸的影响效应取决于微生物可获得的碳源的量.输入地下的植物碳量增加促进土壤微生物呼吸,输入地下的有机质减少则抑制微生物呼吸.④施氮改变了土壤微生物的群落结构组成和底物利用方式,对土壤微生物多样性的影响表现出负效应.⑤施氮提高了β-葡糖苷酶、磷酸酶和大部分糖苷酶的活性,降低了脲酶的活性.迄今为止,施氮对草地土壤微生物特性的影响效应仍存在很大的不确定性,今后的研究中应开展氮输入对草地生态系统影响的长期试验研究、加强对土壤微生物呼吸的影响研究以及合理确定我国草地生态系统可持续发展的氮饱和阈值,并进一步完善和发展测量土壤微生物多样性的新方法.     

马衔山多年冻土与季节冻土区土壤微生物量及酶活性的季节动态

为了研究马衔山多年冻土区和非多年冻土区土壤微生物碳氮、土壤酶活性的差异,选取多年冻土区、季节冻土区和交界区为对象,分析了0~30 cm土层微生物碳氮和转化酶、脲酶、中性磷酸酶、淀粉酶、过氧化氢酶、多酚氧化酶酶活性不同季节的变化特征。结果表明：全氮、总有机碳、微生物量碳氮与多数土壤酶之间呈显著相关关系。在不同区域,土壤微生物碳氮均在0~10 cm含量最高,10~20 cm次之,20~30 cm最低。土壤微生物碳氮在生长季表现为含量逐渐增加,但是多年冻土区与季节冻土区差异不大。土壤酶活性在深度方面表现与微生物碳氮含量变化一致。土壤酶并无的季节变化规律。在多年冻土区,转化酶、多酚氧化酶和磷酸酶活性明显高于非多年冻土区。本研究表明,尽管多年冻土区的植被和土壤总有机碳明显高于非多年冻土区,其土壤微生物碳氮含量相当,且一些土壤酶活性也相当。说明非多年冻土区土壤的生物地球化学相对强度较大。因此,多年冻土退化后可能会导致生态系统的退化。     

Assessing social vulnerability to biophysical hazards in the Kumasi metropolis,Ghana

This study assesses social vulnerability to hazards by analyzing the nature of biophysical hazards, and the characteristics of exposures, susceptibilities and adaptive capacities in Kumasi. A mixed method approach was used to collect primary data from farms and nearby markets. Water, soil, and plant samples were collected and tested for total coliforms and cadmium. Primary dataset was also collected from farmers and vendors using structured interview guide and from key informants through in-depth interviews. The analysis shows that several pressures residing within the coupled social-ecological system are threats to the health and livelihood of the farmers and vendors. The concentration of cadmium and total coliform in irrigation water, soil, and plants exceeded the WHO/FAO acceptable standards. Bio-toxins in plants were also found to be potentially high. However, the degree of exposure to these stressors and the strength of response and adaptive capacities were determined to be dependent on position within the urban agriculture value chain, type of plant, and socio-demographic characteristics. The study concludes that pollution of irrigation water, soil, and plant is an issue of environmental concern in the urban agriculture system and requires concerted efforts of all stakeholders to address. It is recommended that there should be frequent monitoring of the quality of irrigation water, soil, and produce from urban farms and markets to assess their physical, chemical, and microbial properties and possibly initiate remedial measures where necessary. Government policies must also focus on building the resilience of urban farmers and vendors to reduce their vulnerability to biophysical hazards.     

Advances of Soil Microbiology in the Last Decade in China

Soils are fundamental to preservation and sustainability of life-support system on Earth. Soils develop as the most dynamic and complex interface linking atmosphere, hydrosphere, lithosphere and biosphere. Soils harbor enormous diversities of microbial communities as the primary driving forces for global exchanges of matter and energy on our planet. Despite of its profound importance, the invisible soil microbes have for long been underappreciated. In the early 2000s, there has been growing awareness that soil microbiology has attracted huge interest from nonsoil scientists due to the introduction of three domain phylogeny. It is also known as tree of life theory which is widely recognized as the most accurate reflection of the relatedness of all organisms and provides us with a tool to classify and elucidate the largely untapped resource of soil microbial communities. In January 2005, the Department of Earth Sciences of National Natural Science Foundation of China organized a workshop of ‘Soil Biology and Soil Processes’ with focused discussion on soil microbiology research frontiers. The workshop outlined research priorities, cross disciplinary research opportunities, technological needs and potential breakthroughs within soil microbiology. This workshop has witnessed the rapid advances of soil microbiology in soil nutrient transformation, global environmental changes and environmental remediation over the last decade in China. This article will give a brief review on soil microbial researches in the past decade in China, present the status quo of funding system and highlight the challenge and opportunities for future soil microbiology in China.     

Assessment of soil liquefaction potential based on numerical method- a case study of an urban railway project

Paying special attention to geotechnical hazards such as liquefaction in huge civil projects like urban railways especially in susceptible regions to liquefaction is of great importance. A number of approaches to evaluate the potential for initiation of liquefaction, such as Seed and Idriss simplified method have been developed over the years. Although simplified methods are available in calculating the liquefaction potential of a soil deposit and shear stresses induced at any point in the ground due to earthquake loading, these methods cannot be applied to all earthquakes with the same accuracy, also they lack the potential to predict the pore pressure developed in the soil. Therefore, it is necessary to carry out a ground response analysis to obtain pore pressures and shear stresses in the soil due to earthquake loading. Using soil historical, geological and compositional criteria, a zone of the corridor of Tabriz urban railway line 2 susceptible to liquefaction was recognized. Then, using numerical analysis and cyclic stress method using QUAKE/W finite element code, soil liquefaction potential in susceptible zone was evaluated based on design earthquake.     

退化喀斯特植被恢复过程中土壤微生物活性研究——以贵州花江地区为例

退化喀斯特植被的恢复机理一直是近年来土壤学和生态学研究的热点。通过分析退化喀斯特植被恢复过程中土壤微生物数量、土壤生化作用强度、土壤微生物生物量,研究了贵州花江喀斯特高原生态综合治理示范区内土壤微生物活性的变化。结果表明:退化喀斯特植被恢复过程中土壤的微生物活性存在较大差异。随着退化喀斯特植被的恢复,土壤细菌、真菌、放线菌三大微生物数量及微生物总数明显上升,表现为乔木群落阶段〉灌木群落阶段〉草本群落阶段〉裸地阶段,且细菌较真菌和放线菌相比占绝对优势,其在微生物总数中分别占95．95％,93．49％,92．32％和92．48％;各主要生理类群的数量呈上升趋势,且氨化细菌〉固氮菌〉纤维素分解菌〉硝化细菌,土壤生化作用强度增强;土壤微生物生物量碳明显增加,微生物生物量碳与土壤有机碳（P〈0．01）呈显著正相关关系。土壤微生物是表征退化喀斯特植被恢复过程中土壤质量的重要特征之一,反映出随着退化喀斯特植被的恢复土壤质量也在逐渐恢复。      

通辽科尔沁地区土壤微生物群落结构和功能及其影响因素

农业作为通辽科尔沁地区的传统产业,是当地重要经济产业之一.了解土壤微生物群落结构和功能及其影响因素对农作物种植、污染土地修复等具有重要意义.采用Illumina Miseq高通量测序技术、FAPROTAX.1.1功能预测平台等方法,在该区采集71个土壤样品,分析其地球化学特征和微生物群落特征并对微生物群落进行功能预测,同时探讨不同环境因子和空间因子对微生物群落的影响.结果显示:微生物群落由变形菌门（Proteobacteria）（19.77%）、奇古菌门（Thaumarchaeota）（17.85%）、酸杆菌门（Acidobacteria）（17.14%）和放线菌门（Actinobacteria）（15.58%）等构成;功能预测表明该区存在大量活跃的参与氮循环过程的微生物功能群,其中有氧氨氧化功能群为该区的优势微生物功能群.方差分解分析显示,在对微生物群落结构差异的可解释范围内环境因子比空间因子的解释量更大.Mantel检验结果显示,整体微生物群落与土壤pH、EC、TN、C/N及Mg、Na、Sr元素含量显著相关（p < 0.05）.综合分析表明,pH、EC和Sr元素含量是影响通辽科尔沁地区土壤微生物群落结构和功能及多样性的主要环境因素.      

森林土壤微生物与植物碳源的磷脂脂肪酸及其单体同位素研究

森林生态系统的土壤微生物群落组成和活性,是影响生物地球化学循环、有机质代谢和土壤质量的关键因素.磷脂脂肪酸(PLFA)是一类可有效表征活体微生物群落结构的生物标志物,而其单体稳定碳同位素(δ13C)水平对土壤微生物植物碳代谢具有独特的指示作用.本次研究以土壤PLFA为对象,分析了我国位处纬度梯度带上(24°N～47°N...