Spermiogenesis in the yellowfin porgy (Sparus latus Houttuyn), with emphasis on the associated mitochondrion

Transmission electron microscopy of the yellowfin porgy (Sparus latus Houttuyn) spermatozoa ultrastructure showed the spermatozoon as a primitive type made up of the acrosomeless head, the flagellum, and the midpiece, at the periphery of which was a relatively big mitochondrion with more complex structure. It was found that during spermiogenesis, only one relatively big mitochondrion occurred in both the spermatid and the spermatozoon. This is different from other teleost fishes. During spemiogenesis, the mitochondria number is one, and morphology did not change. All these are different from those of other fishes.     

Molecular Characterization,Tissue Distribution and Localization of Larimichthys crocea Kif3a and Kif3b and Expression Analysis of Their Genes During Spermiogenesis

KIF3A and KIF3B are two N-terminal motor proteins belonging to the kinesin-II superfamily that play essential roles in spermiogenesis. To understand the roles played by KIF3 A/3B during spermatogenesis of large yellow croaker Larimichthys crocea, we studied the testis characteristics at different developmental stages of L. crocea, and determined the spatiotemporal expression patterns of kif3a and kif3b during spermiogenesis. Quantitative real-time PCR(qR T-PCR) showed that the overall trends of kif3 a/3 b m RNA abundance during testis development are similar. From stage II to stage V, kif3a/3b m RNA abundances first increased and then fell after reaching a peak at stage IV. Interestingly, the m RNA abundances of both genes at stage V were higher than those at stages II and III. In addition, it is worth of noting that kif3 b m RNA abundance was higher than that of kif3a at all stages. Fluorescence in situ hybridization results revealed that kif3a/3b m RNA abundance dynamics were consistent with the migration of mitochondria, the deformation of nucleus, and the formation of tail. The m RNA hybridization signals of both genes first appeared either around the nuclear periphery or on the side of the nuclei, then appeared at one side of nuclei, and finally were mainly on the tail during spermiogenesis. Our findings contributed to better understanding the molecular mechanisms of spermiogenesis in fish; and suggested that KIF3A and KIF3B may participate in the intracellular transport of mitochondria, nuclear deformation, and the formation of tail during the spermiogenesis in L. crocea.     

CYTOLOGICAL COMPARISON OF GAMETOGENESIS OF SCALLOPS,ARGOPECTEN IRRADIANS AND CHLAMYS FARRERI

ＩＮＴＲＯＤＵＣＴＩＯＮＫｎｏｗｌｅｄｇｅｏｆｇａｍｅｔｏｇｅｎｅｓｉｓｉｎｖａｌｕａｂｌｅｍａｒｉｎｅａｎｉｍａｌｓｉｓｖｅｒｙｉｍｐｏｒｔａｎｔｔｏｃｏｍｍｅｒｃｉａｌｆｉｓｈ ｅｒｙ.Ｇａｍｅｔｏｇｅｎｅｓｉｓｉｎｂｉｖａｌｖｅｓｗａｓｅｘｔｅｎｓｉｖｅｌｙｓｔｕｄｉｅｄａｔｄｉｆｆｅｒｅｎｔｌｅｖｅｌｓ (Ｄｏｈｍｅｎ ,1 983 ;ＤｏｒａｎｇｅａｎｄＬｅＰｅｎｎｅｃ ,1 989ａ,ｂ) .Ｓｃａｌｌｏｐｓａｎｄｏｔｈｅｒｍａｒｉｎｅｂｉｖａｌｖｅｓｈａｖｅｓｉｍｉｌａｒｒｅｐｒｏｄｕｃｔｉｖｅｃｙｃｌｅ…     

EFFECTS OF DIFFERENT COMBINATIONS OF CRYOPROTECTANTS ON SPERMATOZOON VITALITY OF CHINESE SCALLOP,CHLAMYS FARRERI,DURING LOW TEMPERATURE EQUILIBRIUM

The effects of membrane penetrating anti-freezing agents(MPAAs), DMSO(dimethyl sulfoxide),glycerol,EG(ethylene glycol) and methanol in combination with different cryoprotective additives suchas carbohydrates,macromolecules and inorganic compounds on the spermatozoon vitality of Chinesescallop,Chlamys farreri,during 1 h 0℃ equilibrium were investigated.When only MPAAs existed,the detrimental effects of different MPAAs ranked in the following order:DMSO相似文献   

乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构

利用透射电子显微镜观察乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构。结果表明,乌苏里拟鲿精子由头部、中段和尾部组成。头部呈球形或椭圆球形,核前端无顶体,后端有植入窝。中段包括中心粒复合体和袖套。植入窝从细胞核的后端陷入核中。中心粒复合体位于植入窝内,包括近端中心粒和远端中心粒。袖套与细胞核后端相连,袖套中含有丰富的线粒体。尾部主要结构是轴丝,为典型的"9+2"结构,轴丝外两侧为细胞质膜形成的对称性鳍样结构。尾部内无囊泡及其他细胞器。     

CYTOLOGICAL COMPARISON OF GAMETOGENESIS OF SCALLOPS, ARGOPECTEN IRRADIANS AND CHLAMYS FARRERI

Histological characteristics of gametogenesis of two kinds of scallops, gonochoric Chinese scallop, Chlamys farreri and hermaphroditic bay scallop, Argopecten irradians were investigated in this study. Spermatogenesis in C. farreri has different developmental stages: spermatogonia, primary spermatocyte, second spermatocyte, spermatid and spermatozoon. A large number of same developmental stage spermatic cells converge at a definite area of the testis. Premeiotic, previtellogenic and vitellogenic oocytes can be found during oogenesis in C. farreri, where oocyte distribution is obviously irregular. The A. irradians gonad consists of two different parts in one individual: one part functions as testis, the other as ovary. Between these two parts is a special appearance area, where a large number of spermatic cells are bound with two layers of acellular substance with many oocytes in it.     

Effects of different combinations of cryoprotectants on spermatozoon vitality of Chinese scallop,Chlamys farreri,during low temperature equilibrium

The effects of membrane penetrating anti—freezing agents (MPAAs), DMSO (dimethyl sulfoxide), glycerol, EG (ethylene glycol) and methanol in combination with different cryoprotective additives such as carbohydrates, macromoleules and inorganic compounds on the spermatozoon vitality of Chinese scallop,Chlamys farreri, during 1 h 0 °C equilibrium were investigated. When only MPAAs existed, the detrimental effects of different MPAAs ranked in the following order: DMSO<methanol<EG<glycerol. When carbohydrates were added into MPAAs solution, 5% glucose caused larger decrease of spermatozoon vitality than 2.4% lactose. 5% glucose or 2.4% lactose in 7.5% glycerol caused complete damage. 10% yolk was best in maintaining the spermatozoon vitality except when used in combination with 10% methanol. 10% milk significantly decreased spermatozoon vitality in EG and methanol and enhanced its vitality in glycerol, but did not significantly influence it in DMSO. Glycine MPAAs ranked in the following order. 10% yolk <10% yolk +2.4% lactose <2.4% lactose <2.0% citrate <10% milk <10% milk +2.0% citrate <5% glucose <80mmol/L glycine +55 mmol/L NaHCO₃. Contribution No. 2434 from the Institute of Oceanology, Chinese Academy of Sciences.     

FURTHER OBSERVATION OF THE SPERMATOZOA OF LEFT-EYE FLOUNDER PARALICHTHYS OLIVACEUS BY ELECTRONIC MICROSCOPY

ＩＮＴＲＯＤＵＣＴＩＯＮＢｉｌｌａｒｄ ( 1 986)ｓｔｕｄｉｅｄｔｈｅｓｐｅｒｍａｔｏｚｏｏｎｕｌｔｒａｓｔｒｕｃｔｕｒｅｓｉｎｔｅｌｅｏｓｔｓａｎｄｆｏｕｎｄｄｉｓｔｉｎｃｔｄｉｆｆｅｒｅｎｃｅｓａ ｍｏｎｇｄｉｆｆｅｒｅｎｔｓｐｅｃｉｅｓ.Ｓｔｕｄｙｏｎｓｐｅｒｍａｔｏｚｏｏｎｍｏｒｐｈｏｌｏｇｙａｎｄｆｕｎｃｔｉｏｎｉｓａｃｈａｌｌｅｎｇｅｂｅｃａｕｓｅｏｆｔｈｅｓｐｅｒｍ’ｓｓｐｅｃｉａｌｅｎｖｉｒｏｎｍｅｎｔ (Ｇａｇｅｅｔａｌ.,1 998;ＷａｎｇａｎｄＺｈａｎｇ,1 999) .Ｓｉｎｃｅｌｅｆｔ ｅｙ…     

湛江沿海鱼类补遗

增补湛江市沿海鱼类种类有51种,隶属于11目39科45属,其中软骨鱼类8种;硬骨鱼类43种,(鲈形目30种)。现知湛江市沿海鱼类有222种。     

EXPERIMENTAL STUDIES ON THE ROLE OF PLANKTIVOROUS FISHES IN THE ELIMINATION OF MICROCYSTIS BLOOM FROM DONGHU LAKE USING ENCLOSURE METHOD

The hypertrophic subtropic Donghu Lake's dense water bloom(of mainly Microcystis Anaabaena andOscillatoria)that occurred annually from the beginning of the 1970s, has disappeared since 1985. The infiuenceof planktivorous fishes (silver and bighead carps) on the water bloom was studied for three years usingthe enclosure method. The enclosures stocked densely with bighead and/or silver carp were free of waterbloom during the experimental period. The water bloom that appeared in the fish-free enclosures was completelyeliminated in 10-20 days by introduction of silver and/or bighead carp(grass carp was not effectivein controlling water bloom).This study showed clearly that grazing pressure by planktivorous fishes is a keyfactor in eliminating water bloom from the lake.     

Spatial and seasonal distribution of intertidal macrobenthos with their biomass and functional feeding guilds in the Naf River estuary,Bangladesh

The Naf River estuary is one of the most productive ecological ecosystems in Bangladesh providing an important feeding area for fishes and other aquatic animals. However, detailed information on macrobenthic communities is rarely available in this area. Our study focused on the seasonal and spatial variability of macrobenthic community structure with their biomass and functional guilds. In total, fortyseven taxa were identified under nine major groups and seven functional feeding guilds. Among macrobenthic taxa, Polychaeta was dominant in both seasons and all over the estuary contributing 60% of total benthos.The mean abundance of macrobenthos was higher during the pre-monsoon(2 972±1 994 inds./m~2) than the monsoon(1 572±361 inds./m~2) being maximum at mid-estuary region and minimum at upper and lower estuary regions. Of the diversity indices, density(P=0.01, P=0.003), the number of taxa(P=0.000 3,P=0.000 9) and Margalefs Index(P=0.000 4, P=0.001) of macrobenthos were significantly different among stations and seasons. Shannon-Weiner index(P=0.009, P=0.12) and Pielou's evenness index(P=0.03,P=0.14) had significant differences among stations but not among seasons. Cluster analysis suggested that distribution of macrofauna is strongly influenced by seasonality. Among the feeding types, carnivorous were found to be dominant at the upper and mid-estuarine regions, and herbivorous were at lower estuarine regions. Carnivorous species were dominant in both seasons. Omnivores have shown to be the most abundant feeding type in terms of biomass though herbivores were highest during monsoon.     

Ultrastructure developments during spermiogenesis in Polydora ciliata (Annelida: Spionidae), a parasite of mollusca

Spionid worms of Polydora ciliata inhabit the shells of many commercially important bivalves and cause disease in molluscan aquaculture. Their sperm structure is closely related to their fertilization method. To give an insight into the sperm structure and spermatogenesis, ultrastructure details of the subcellular components of germ cells during spermiogenesis of Polydora ciliata are detected by transmission electron microscopy (TEM). In P. ciliata, during spermiogenesis, chromatin is regularly arranged as dense fibrils and becomes more condensed when the nucleus elongates. Microtubules do not surround the nucleus during its elongation. The Golgi phase is characterized by the formation of proacrosomal granules within the Golgi apparatus. The proacrosomal granules fuse to form a single, spherical acrosomal vesicle that migrates to the anterior pole of the cell. At the time of nuclear condensation, mitochondria become reduced in number but increased in size, causing deep indentation at the base of the nucleus. The mid-piece has a few mitochondria. The cap phase includes the spreading of the acrosomal granule over the surface of the nucleus of the differentiating spermatid. The acrosomal phase of spermiogenesis is typically associated with changes in the shape of the nucleus, acrosome and tail. The relationship of sperm ultrastructure to spermiogenesis in spionidae species was discussed.     

东闯金矿自然金矿物学研究

东闯金矿自然金以其不依赖硫化物而存在的特殊产状、巨粒的形态有别于小秦岭其他金矿的自然金。本文在叙述成矿地质背景的基础上,着重论及了它的产状、化学成分、物理性质等特征;给出了自然金X射线衍射数据;提供了完整的自然金反射率色散曲线;计算了自然金的颜色指数并指出了它们与成色之间的关系;同时也对该矿区金在热液中搬运、沉淀方式作了探讨。     

提高鳜鱼催产率受精率和苗种成活率的试验

在常规催产剂中加入适量的Ａ型添加剂，可使鳜鱼的催产率提高１０％以上，受精率提高５％以上。使用１５×１０－６的福尔马林，可有效地杀灭寄生于鳃及体表的寄生虫和细菌，从而大大提高苗种成活率。     

广西防城沿海鱼类初步调查

广西防城沿海鱼类初步调查有115种,隶属于14目63科91属。软骨鱼类有2目3科4属5种,占总数的4.3％。硬骨鱼类有12目60科87属110种,占总数的95.7％,其中鲈形目鱼类占优势,有68种。暧水性鱼类99种,占总数的86％;暧温性鱼类16种,占总数的14％,无冷温性鱼类。     

关于城市地震

本文提出了一种新观点，即大城市的出现对地震有激发作用，列举了被认为是城市激发的震例，讨论了城市地震发生的机理，其中仅从重力荷载这一个方面的定量讨论中也可以得出：如果其它外力对地震有激发作用，那么城市激发地震的现象更值得重视，还讨论了涉及到城市激发地震的其它问题．     

The complete mitochondrial genome of Parachiloglanis hodgarti and its phylogenetic position within Sisoridae

The complete mitogenome of Parachiloglanis hodgarti was sequenced and characterized, which is the first mitogenome of the genus Parachiloglanis within Sisoridae. The mitogenome is 16 511-bp long and included 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and one control region. The genome composition was A+T biased (58.64%) and exhibited a positive AT-skew (0.095) and a negative GC-skew (?0.283). Compared with other Sisoridae fishes, the characteristics of nucleotide skews, codon usage, and amino acid usage of the P. hodgarti mitogenome are more similar to those of non-Glyptosternoid fishes. Furthermore, the phylogenetic trees show that Sisoridae fishes fall into four major clades and P. hodgarti (Clade I) is basal to the Sisoridae family, forming a sister clade to the Glyptosternoids (Clade IV). The topological structures constructed in this study raised doubts over the traditional classification system. These results will help better understand the features of the P. hodgarti mitogenome and provide a reference for further phylogenetic research on Sisoridae species.

     

鲢、鳙溶血性腹水病研究

报道了鲢、鳙鱼溶血性腹水病的病原和防治方法。研究结果表明，该病无病毒性病原存在，病原为嗜水气单胞菌嗜水亚种（Aeromonashydrophilasubsp.hydrophila）。采用作者研制的鱼康乐（Y·K·L）进行治疗。治愈率达86．5‰。     

Evidence for the role of KIF17 in fish spermatid reshaping: expression pattern of KIF17 in Larimichthys polyactis spermiogenesis

Journal of Oceanology and Limnology - The homodimeric kinesin-2 protein KIF17 functions in intracellular transport and spermiogenesis in mammals. However, its role in fish spermiogenesis has not...     

A comparison between benthic gillnet and bottom trawl for assessing fish assemblages in a shallow eutrophic lake near the Changjiang River estuary

Two fishing methods including gillnetting and trawling to estimate attributes of fish assemblage were compared in Dianshan Lake from August 2009 to July 2010. Species composition dif fered significantly between the gears, with four significant contributors in gillnet catches and one in trawl catches. Trawling collected more proportions of benthic species by number and biomass than gillnetting. Size distribution was significantly influenced by fishing technique; gillnetting captured relatively less small-sized fishes and trawling captured less large-sized individuals. Trawling produced species richness closer to the one expected than gillnetting. On the whole, trawl catch was a quadratic polynomial function of gillnet catch and a significantly negative correlation was found between them, both of which varied as dif ferent polynomial functions of temperature. However, trawl and gillnet catches were significantly correlated only in one of five month groups. It is concluded that single-gear-based surveys can be misleading in assessments of attributes of fish assemblages, bottom trawling is a more ef fective gear for assessing fish diversity than benthic gillnetting, and using gillnet catches as an indicator of fish density depends on fishing season in the lake.