Influence of Vegetation Characteristics on Soil Denitrification in Shoreline Wetlands of the Danjiangkou Reservoir in China

Soil denitrification in reservoir shoreline wetlands is an important process for removing excess inorganic nitrogen from upland runoff and controlling eutrophication in aquatic ecosystems. As yet, little is known about the influence of vegetation characteristics on the soil denitrification potential in reservoir shoreline wetlands, although vegetation can affect both denitrifying bacteria and soil properties. In this study, we measured the spatial variability of denitrification enzyme activity (DEA) using acetylene block method in shoreline wetlands of the Danjiangkou Reservoir, a water source of the South‐to‐North Water Transfer Project in China. Results indicated that DEA ranged from 0.001 to 2.449 µg N (N₂O) g^?1 h^?1, with a mean of 0.384 µg N (N₂O) g^?1 h^?1. DEA varied significantly among five representative plant communities and the highest DEA (0.248–2.449 µg N (N₂O) g^?1 h^?1) was observed in the Polygonum hydropiper community. Plant biomass and vegetation cover were significantly and positively related to DEA and together explained 44.2% of the total variance. These results suggest that vegetation characteristics should also be considered in assessing soil denitrification capacity and restoring shoreline wetlands for nitrogen pollution removal in the Danjiangkou Reservoir after dam heightening.     

三峡支流澎溪河消落带落干初期N2O释放与反硝化特征初探

水库近岸湿地(消落带)土壤N2O释放和反硝化作用是消落带氮的生物地球化学过程的重要组成部分.以三峡水库支流澎溪河高阳平湖库湾消落带为研究对象,于2013年落干初期,采用C2H2抑制-原状土柱培养法研究该处自然植被恢复区、农耕区和对照组等不同土地类型土壤的N2O释放速率和反硝化速率,并测定了土壤p H值、氧化还原电位、温度、有机质、总氮、铵态氮、硝态氮和土壤孔隙含水量等环境指标.结果表明,自然植被恢复区土壤N2O释放速率为9.88±6.49 g N/(hm2·d),反硝化速率为58.94±52.84 g N/(hm2·d);农耕区土壤N2O释放速率和反硝化速率分别为7.71±4.44和30.70±25.68 g N/(hm2·d).不同土地类型间N2O释放速率差异显著,落干初期土壤氧含量、含水量及氮含量对不同土地类型N2O释放和反硝化作用影响明显.土壤氧含量的升高促进了自然植被恢复区的N2O释放,并在一定程度上抑制了该区域反硝化作用.农耕区土壤含水量高于自然植被恢复区,可能致使N2O释放速率低于自然植被恢复区,而反硝化速率高于自然植被恢复区.消落带土壤氮含量降低同反硝化速率降低有一定联系.     

Nitrogen Removal from Landfill Leachate Using an Infiltration Bed Coupled with a Denitrification Barrier

Treatment of nitrogen in landfill leachate has received considerable attention recently because of the relatively low levels at which some nitrogen species (i.e., NH3) can be toxic to aquatic life forms. This study reports on the results of a three-year, pilot-scale field trial demonstrating the use of infiltration bed and nitrate barrier technology to achieve nitrogen removal in landfill leachate. The infiltration bed comprises an unsaturated sand layer overlying a saturated layer of waste cellulose solids (sawdust), which acts as a carbon source for heterotrophic denitrification. When loaded at a rate of 1 to 3 cm/day, the infiltration bed was successful at lowering leachate inorganic nitrogen (NH₄⁺+ NO₃^-) levels averaging 24.8 mg/L N by 89%, including 96% in the third year of operation. The surface water discharge criteria for un-ionized ammonia (NH3) were met on all occasions in the treated leachate during the second and third years of operation. Nitrogen attenuation is presumed to occur by a two-step process in which leachate NH₄⁺ is first oxidized to NO₃^- in the unsaturated sand layer and then is converted to nitrogen gas (N₂) by denitrification occurring in the underlying sawdust layer. Mass balance calculations suggest that the sawdust layer has sufficient carbon to allow denitrification to proceed for long periods (1.0 to 30 years) without replenishment. Because this technology is simple to construct and is relatively maintenance free, it should be attractive for use at smaller landfills where the installation of conventional treatment plants may not be feasible.     

Sources and sinks of nutrients in a New Zealand hill pasture catchment III. Nitrogen

A study was made of the nitrogen (N) inputs to, and exports from, a stream draining a pasture catchment near Hamilton, New Zealand, in order to plan measures for minimizing N losses to natural waters. An estimated 7 kg N ha^?1 was exported from the catchment during 1981 of which 86 per cent was in reduced forms (Kjeldahl-N, TKN) and the remainder as nitrate-N (NO₃-N). Virtually all of the reduced N inputs came from saturated overland flow whereas NO₃-N inputs were dominantly subsurface derived. The TKN exported by individual storm events could be predicted (R² = 0.97) from peak flow and from the peak flow rate in the seven days preceding the storm. A TKN balance for eight events showed that except for large floods (return period approximately a year) the stream system was a net sink for TKN. During large floods, scouring of the organic rich seepage areas resulted in the stream system itself being a net source of TKN. Microbial assays for nitrification and denitrification activity indicated that the main nitrate source was the well-aerated greywacke and ash soils and that the permanently saturated seepage zones were a significant nitrate sink. An in-stream nitrate addition experiment showed that up to 20mg N m^?2 h^?1 was removed from the stream. Simultaneous measurements of in situ denitrification activity demonstrated that only about 1 per cent of this removal could be accounted for by denitrification. It was inferred that plant uptake was responsible for the remainder. Retention of near-stream seepage areas is suggested as a measure for minimizing NO₃-N export, whilst removal of stock from seasonally saturated areas during periods of saturatior should reduce soil loss and hence TKN inputs to the stream.     

太湖梅梁湾水土界面反硝化和厌氧氨氧化

运用无扰动芯样实验室内流动培养、稳定同位素示踪、同位紊气态产物测定及同位素配对技术,对太湖梅梁湾北部到南部的4个梯度样点的水土界面反硝化和厌氧氨氧化速率进行研究.结果表明,梅梁湾内及湾外开敞湖区4个样点的水土界面反硝化脱氮速率为(46.36±13.26)-(16.34±22,74)μmol/(m~2·h),厌氧氨氧化脱氮速率为(7.50±2.21)-(2.05±2.90)～mol/(m~2.b).梅梁湾北部河口区水土界面总脱氮能力明显高于梅梁湾南部及开敞湖区.通过对脱氮过程的进一步研究发现.北部脱氮过程主要以上覆水硝酸盐为底物的非耦合反硝化过程(D_w)为优势过程,而梅梁湾外开敞湖区则以沉积物硝化过程耦合控制的反硝化(D_n)为主.影响D_n、D_w在反硝化中比重的主要因素是沉积物溶氧侵蚀深度和上覆水NO_3~-.浓度的差异;梅梁湾厌氧氨氧化脱氮比例占总脱氮比例为12%-14%,湾外开敞湖区则占11%,影响其比例差异的主要因子是反硝化强度的大小及其反硝化中间产物--亚硝酸盐含量的差异.     

Soil Budget,Net Export,and Potential Sinks of Nitrogen in the Lower Oglio River Watershed (Northern Italy)

A nitrogen mass balance, realized for the lower Oglio River basin (Po River Plain, northern Italy), suggested an elevated impact of agricultural activities in this watershed. Livestock manure, synthetic fertilizers, biological fixation, atmospheric deposition, and wastewater sludge contributed 51, 34, 12, 2, and 1% of total N (TN) input, respectively (basin average 450 kg N ha^?1 arable land (AL) year^?1, overall input 100 115 t N year^?1). Crop uptake, ammonia volatilization and denitrification in soils contributed 65, 21, and 14%, respectively, of TN output (basin average 270 kg N ha^?1 AL year^?1, overall output 60 060 t N year^?1). N inputs exceeded outputs by 40 056 t N year^?1, resulting in a basin average surplus of about 180 kg N ha^?1 AL year^?1. About 34% of the N surplus was exported annually from the basin while the remaining amount (about 26 800 t N year^?1) underwent other unaccounted for processes within the watershed. The relevance of nitrogen removal via denitrification in aquatic compartments within the watershed was evaluated. Denitrification in the secondary drainage network can represent a relevant nitrogen sink due to great linear extension (over 12 500 km), with estimated nitrogen loss up to 8500 t N year^?1. Denitrification in the riverbed and in perifluvial wetlands have the potential to remove only a small fraction of the nitrogen surplus (<3%). Evidence suggests the relevance of groundwater as a site of nitrogen accumulation.     

Natural and anthropogenic nitrogen uptake by bloom-forming macroalgae

The frequency and duration of macroalgal blooms have increased in many coastal waters over the past several decades. We used field surveys and laboratory culturing experiments to examine the nitrogen content and delta(15)N values of Ulva and Gracilaria, two bloom-forming algal genera in Narragansett Bay, RI (USA). The northern end of this bay is densely populated with large sewage treatment plant nitrogen inputs; the southern end is more lightly populated and opens to the Atlantic Ocean. Field-collected Ulva varied in delta(15)N among sites, but with two exceptions had delta(15)N above 10 per thousand, reflecting a significant component of heavy anthropogenic N. This variation was not correlated with a north-south gradient. Both Ulva and Gracilaria cultured in water from across Narragansett Bay also had high signals (delta(15)N= approximately 14-17 per thousand and 8-12 per thousand, respectively). These results indicate that inputs of anthropogenic N can have far-reaching impacts throughout estuaries.     

Benthic denitrification and organic matter mineralization in intertidal flats of an enclosed coastal inlet, Ago Bay, Japan

Denitrification (as N(2) flux) and organic matter mineralization (as O(2) uptake) were simultaneously measured in the same set of core sediments from a natural sandy and a constructed muddy tidal flat of Ago Bay, Japan. Denitrification rates at both tidal flats fluctuated between ca. 2-20 micromol N(2)m(-2)h(-1) without showing a clear seasonal pattern, and appeared to be substrate limited as NO(3)(-) enrichment (final concentration ca. 225 microM) caused prompt and similar enhancements of ca. 10-folds. Organic matter mineralization rates were markedly higher at constructed muddy flat compared to those of natural sandy flat, especially in summer, and exhibited pronounced temperature dependent (p<0.01) seasonality for both tidal flats. O(2) uptake rates were generally ca. 2-3 order greater than respective denitrification rates indicating dominance of mineralization processes over N(2) losses.     

Simulating estuarine nitrous oxide production by means of a dynamic model

We describe a dynamic model developed from a commercially available modeling package (ECoS-III) to simulate estuarine dissolved inorganic nitrogen (DIN) dynamics, and consequent N(2)O production and atmospheric flux on the timescale of tidal cycles. Simulated model state variables were NH(4)(+), NO(3)(-) and N(2)O concentrations, and salinity. Model outputs were evaluated through comparison with summer field data for the Tyne estuary, UK. The model adequately reproduced the observed axial profiles of NH(4)(+), NO(3)(-) and N(2)O concentrations. Nitrification was shown to be the dominant N(2)O source and estimates of the ratios nitrification to DIN load and N(2)O emission to DIN load are considerably lower than the corresponding values adopted in global scale models of estuarine N(2)O emissions based on DIN transformations. Hence our results are consistent with the requirement imposed by atmospheric N(2)O growth rate constraints that the amount of atmospheric N(2)O arising from agriculturally related sources, including estuarine transformations of N, be revised downward.     

Reservoirs are hotspots of nitrogen cycling in peatland catchments

This study presents input–output budgets of total dissolved nitrogen (TDN), dissolved organic N (DON) and dissolved inorganic N (DIN) for a reservoir in a peatland catchment in the south Pennines (UK). This site receives high levels of atmospheric inorganic N deposition, in the range of 26 kg N ha^?1 yr^?1. The results show that the reservoir retains ~21 to 31% of the annual TDN input (8806 ± 741 kg N). Approximately 39 to 55% of DON (3782 ± 653 kg N) and 6 to 13% of DIN (5024 ± 349 kg N) were retained/processed. A long water retention time (104 days), average annual pH of 6.5, high concentrations of DIN in the reservoir water and a deep water column suggest that denitrification is potentially a key mechanism of N retention/removal. The results also demonstrate that DON is potentially photodegraded and utilized within the reservoir, particularly during the summer season when 58 to 80% of DON input (682 ± 241 kg N) was retained, and a net export of DIN (~34 kg N) was observed. The findings therefore suggest that DON may play a more crucial role in the biogeochemistry of peat‐dominated acid sensitive upland freshwater systems than previously thought. Reservoirs, impoundments and large lakes in peatland catchments may be important sites in mediating downstream N transport and speciation. Copyright © 2016 John Wiley & Sons, Ltd.     

A method to determine which nutrient is limiting for plant growth in estuarine waters--at any salinity

A method, utilising overlaid graphs for nutrients vs salinity, was developed in order to determine which nutrient is limiting for plant growth in estuarine waters-at any salinity. Dissolved inorganic nitrogen (DIN=NO(3)(-)+NO(2)(-)+NH(4)(+)) and o-phosphate (PO(4)(-)) are the main forms of N and P that are readily bio-available for plant growth in waters and these have a Redfield atomic ratio of N:P=16:1 (i.e. aquatic plants absorb N and P in the average ratio of 16 atoms of N to 1 atom of P). Graphs are prepared for (i) DIN vs salinity and (ii) o-phosphate vs salinity with the vertical scales for DIN and o-phosphate set at a ratio of N:P=16:1; when these graphs are overlaid on each other then the lowermost trendline denotes the limiting nutrient for plant/algal growth-at any salinity. The graphs also indicate the extent by which one or other of the nutrients is limiting--at any salinity. Furthermore, if there is a transition from P to N limitation somewhere along the salinity gradient, then this occurs at the salinity where the trendlines intersect. The concept was applied to three estuaries in the southeast of Ireland and the results show that, in all of these circumstances, P is the limiting nutrient throughout--except for the higher salinities (i.e. salinities 30 per thousand), where either (i) N and P may become equally limiting at salinity approximately 35 per thousand or (ii) N may become limiting at salinity 30 per thousand. Overlaid nutrients vs salinity graphs were also used to demonstrate that, in the estuaries in southeast Ireland, carbon (as dissolved inorganic carbon, DIC=CO(2)+H(2)CO(3)+HCO(3)(-)+CO(3)(2-)) is not the limiting nutrient--at any salinity.     

Quantifying nitrogen cycling beneath a meander of a low gradient,N‐impacted,agricultural stream using tracers and numerical modelling

In watersheds impacted by nitrate from agricultural fertilizers, nitrification and denitrification may be decoupled as denitrification in the hyporheic zone is not limited to naturally produced nitrate. While most hyporheic research focuses on the 1–2 m of sediment beneath the stream bed, there are a limited number of studies that quantify nitrogen (N) cycling at larger hyporheic scales (10s of metres to kms). We conducted an investigation to quantify N cycling through a single meander of a low gradient, meandering stream, draining an agricultural watershed. Chemistry (major ions and N species) and hydrologic data were collected from the stream and groundwater beneath the meander. Evidence indicates that nearly all the shallow groundwater flowing beneath the meander originates as stream water on the upgradient side of the meander, and returns to the stream on the downgradient side. We quantified the flux of water beneath the meander using a numerical model. The flux of N into and out of the meander was quantified by multiplying the concentration of the important N species (nitrate, ammonium, dissolved organic nitrogen (DON)) by the modelled water fluxes. The flux of N into the meander is dominated by nitrate, and the flux of N out of the meander is dominated by ammonium and DON. While stream nitrate varied seasonally, ammonium and DON beneath the meander were relatively constant throughout the year. When stream nitrate concentrations are high (>2 mg litre^?1), flow beneath the meander is a net sink for N as more N from nitrate in stream water is consumed than is produced as ammonium and DON. When stream nitrate concentrations are low (<2 mg litre^?1), the flux of N entering is less than exiting the meander. On an annual basis, the meander hyporheic flow serves as a net sink for N. Copyright © 2007 John Wiley & Sons, Ltd.     

东南丘陵山区深水水库两种浮床植物脱氮效率对比研究

为探究东南丘陵山区深水水库中生态浮床技术深度脱氮的效率及管理措施,以大型山谷型深水水库千岛湖为例,选取湿生植物空心菜(Ipomoea aquatica)和水生植物粉绿狐尾藻(Myriophyllum aquaticum),采用生态浮床技术,开展了原位模拟实验对比研究,探究不同营养盐浓度和光强下两种植物的生长状况与氮素去除效率。结果发现:(1)空心菜长势受营养盐和光照条件影响明显,添加氮磷后(TN=2.37 mg/L,TP=0.046 mg/L)的空心菜生物量是原位水体(TN=0.66 mg/L,TP=0.028 mg/L)的1.6倍,适当遮光有助于浮床植物生长,40%遮光条件下空心菜的生物量是不遮光条件下的1.5倍;而粉绿狐尾藻生长受营养盐和光照条件影响均较小。(2)空心菜对于水体氮素净化能力显著高于粉绿狐尾藻,在最佳条件下空心菜和粉绿狐尾藻对氮素的去除效率分别达到213.30和44.23 mg/(m²·d)。(3)空心菜去除氮主要以植物同化作用为主,占70%以上TN去除量,40%遮光环境通过明显提升空心菜同化吸收氮量和根系反硝化速率增强了氮的去除能力;粉绿狐尾藻同化吸收和反硝化脱氮作用各占50%左右,以遮光75%下脱氮效果最好。本研究表明,采用生态浮床技术能够强化深水水库的脱氮能力,空心菜更适合在氮浓度较高的水体生长,夏季为空心菜和粉绿狐尾藻浮床分别进行40%和75%的遮光处理将有更好的水质净化效果。因此,在滨岸湿地匮乏的深水水库实施多种植物搭配的生态浮床技术强化水体脱氮作用是一种行之有效的水质改善方法。     

Removal mechanisms of nitrogen in waste stabilization ponds

The aim of this research work was to determine the major nitrogen transformation and removal mechanisms in primary and maturation ponds. To accomplished this objective, nitrogen mass balance in waste stabilization pond system was determined using a dynamic mathematical model in order to elucidate the biological nitrogen transformation mechanisms that are effective for removal of nitrogen in this pond system. Results show that nitrogen removal efficiency in a primary facultative pond unit was 13.2%, which was largely due to net loss of organic nitrogen to sediments (9.76%) and denitrification (3.42%). On the other hand, maturation pond removed 15.2% of nitrogen received in the influent with denitrification (13.55%) being the major pathway for nitrogen removal. Ammonia volatilization was not a predominant mechanism for nitrogen removal in both primary facultative and maturation ponds. The major nitrogen transformation routes were mineralization and ammonia uptake in the primary facultative pond, but ammonia uptake by microorganisms was a predominant nitrogen transformation mechanism in maturation pond.     

Nitrogen loading to Pleasant Bay, Cape Cod: application of models and stable isotopes to detect incipient nutrient enrichment of estuaries

To test and refine methods to detect nutrient enrichment and resulting eutrophication, we applied the Waquoit Bay nitrogen loading model (NLM) and Estuarine loading model (ELM) to estuaries of Pleasant Bay that receive increasing but low N loads (25-199 kg N ha(-1) yr(-1)) from land. Contributions of wastewater to these estuaries increased from 7% to 63% as N loads increased, and modeled estimates of dissolved inorganic nitrogen in the water were within approximately 27% of measured values. N isotopic signatures in suspended and benthic organic matter and in tissue of quahogs increased as wastewater contributions to N loads increased, with clams approximately 4 per thousand heavier than organic matter, indicating that even at these low N loads, N from land-derived sources moved detectably up the food web. These results extend the application of NLM and ELM to detect incipient levels of N enrichment and demonstrate that these models can be used in conjunction with isotope measurements as the basis for food web analyses in a system exposed to relatively lower N loads than previously studied.     

Methane and nitrous oxide fluxes in the polluted Adyar River and estuary, SE India

We measured dissolved N(2)O, CH(4), O(2), NH(4)(+), NO(3)(-) and NO(2)(-) on 7 transects along the polluted Adyar River-estuary, SE India and estimated N(2)O and CH(4) emissions using a gas exchange relation and a floating chamber. High NO(2)(-) implied some nitrification of a large anthropogenic NH(4)(+) pool. In the lower catchment CH(4) was maximal (6.3+/-4.3 x 10(4)nM), exceeding the ebullition threshold, whereas strong undersaturation of N(2)O and O(2) implied intense denitrification. Emissions fluxes for the whole Adyar system approximately 2.5 x 10(8) g CH(4)yr(-1) and approximately 2.4 x 10(6)gN(2)O yr(-1) estimated with a gas exchange relation and approximately 2 x 10(9) g CH(4)yr(-1) derived with a floating chamber illustrate the importance of CH(4) ebullition. An equivalent CO(2) flux approximately 1-10 x 10(10)gy r(-1) derived using global warming potentials is equivalent to total Chennai motor vehicle CO(2) emissions in one month. Studies such as this may inform more effective waste management and future compliance with international emissions agreements.     

沉水植物水槽对农村水体净化效果与机制的模拟

实验设计中试水槽装置,模拟研究2种沉水植物轮叶黑藻(Hydrilla nerticillata)和苦草(Vallisneria natans)对农村水体的净化效果,并探讨沉水植物在水体氮、磷去除中的作用.结果显示,沉水植物系统均能显著降低水体中总氮、铵态氮、硝态氮和磷酸盐浓度,去除率分别达到50.5%、84.4%、41.9%和64.6%,且轮叶黑藻组选择20 g/m2,苦草组选择40 g/m2为适种密度.水体流经各串联单元时氮、磷去除负荷存在显著差异,其中总氮、硝态氮、磷酸盐浓度在有植物单元高于无植物单元,铵态氮浓度相反,结合单元内溶解氧浓度、硝化与反硝化细菌总数表明,有植物单元可显著发生反硝化作用,无植物单元可显著发生硝化作用,有无植物串联单元更有利于氮的去除.     

Increase of nitrous oxide flux to the atmosphere upon nitrogen addition to red mangroves sediments

Response of nitrous oxide N20 sediment/air flux to nitrogen addition was assessed in mangrove (Rhizophora mangle) sediments. Fluxes were enhanced with both ammonium and nitrate loading. Greatest fluxes (52 micromol m(-2) h(-1)) were obtained with ammonium addition and saturation was achieved with additions of 0.9 mol m(-2). Maximum flux following ammonium addition was 2785 times greater than control plots and 4.5 times greater during low tide than with equivalent ammonium addition at high tide. Nitrate enrichment resulted in exponential growth, with maximal mean flux of 36.7 micromolm(-2) h(-1) at 1.9 molm(-2); saturation was not achieved. Differential response to ammonium and nitrate, and to tide and elevation, indicate that microbial nitrification is responsible for most of the observed gas flux. Mangrove sediments constitute an important source of global atmospheric N20 and increases in nitrogen loading will lead to significant increases in the flux of this atmospherically active gas.     

Extreme supersaturation of nitrous oxide in a poorly ventilated Antarctic lake

Lake Bonney, a permanently ice-covered Antarctic lake, has a middepth maximum N2O concentration of 41.6 micromoles N (>580,000% saturation with respect to the global average mixing ratio of N2O) in its east lobe, representing the highest level yet reported for a natural aquatic system. Atmospheric N2O over the lake was 45% above the global average, indicating that this lake is an atmospheric source of N2O. Apparent N2O production (ANP) was correlated with apparent oxygen utilization (AOU), and denitrification was not detectable, implying that nitrification is the primary source for this gas. The slope of a regression of ANP on AOU revealed that potential N2O production per unit of potential O2 consumed in the east lobe of Lake Bonney is at least two orders of magnitude greater than reported for the ocean. The maximum yield ratio for N2O [ANP/(NO2(-) + NO3-)] in Lake Bonney is 26% (i.e. 1 atom of N appears in N2O for every 3.9 atoms appearing in oxidized N), which exceeds previous reports for pelagic systems, being similar to values from reduced sediments. Areal N2O flux from the lake to the atmosphere is >200 times the areal flux reported for oceanic systems; most of this gas apparently enters the atmosphere through a small moat that occupies approximately 3% of the surface of the lake and exists for approximately 10 weeks in summer.     

利用不同物候期水生植物配置提高浮床人工湿地的除氮效果

为验证利用不同物候期水生植物配置提高碳氮比失衡湿地脱氮能力的可行性,设计了向水芹菜(Oenanthe javanica)浮床人工湿地系统中添加轮叶黑藻(Hydrilla verticillata)的实验,研究了在秋、冬季低温条件下,暖季型植物分解释碳对系统氮去除效果的影响.结果表明,添加轮叶黑藻显著提高了系统水体碳氮比,促进反硝化作用,提高总氮和硝态氮的去除率.在4个周期中,与对照组相比,物候期组合型浮床人工湿地系统的总氮去除率分别提升23.03%、10.90%、18.55%和22.93%,硝态氮去除率分别提升38.28%、20.74%、17.87%和17.06%.由此可见,利用暖季型和冷季型植物配置提高碳氮比失衡湿地氮去除率是可行的.