Abstract: Determination of sampling layer, rough machining size fraction of samples and indicator elements are important contents for experimental study of soil geochemical survey of valley,directly related to the validity of the soil geochemical anomalies for using soil geochemical survey of valley to mine searching.Studied the weathering soil from limestone, basalt and shales sandstone, dried soil geochemical samples of three horizons(epipedon,illuvial horizon,parent material horizon) at room temperature.Samples sieving for Shenyang layer vibrating screen of 20,40,60 and 80 meshs. determined element content of samples.An experiment study on soil mechanical composition and element content of soil measured for three horizons of four size fractions (-20 ～+40,-40～+60,-60～+80,-80) in the Puan-Qinglong antimony -gold exploration area,determined upper part of illuvial horizon for sampling layer in the Puan-Qinglong antimony -gold exploration area,rough machining size fraction of samples is from 20 to 40 mesh fraction.The Au,Sb,As,Cu elements had been mainly considered the indicator elements. Results to ensure that ,soil geochemical anomalies from soil geochemical survey of valley are effective for geological prospecting in the Puan-Qinglong antimony -gold exploration.