| 黄鳍金枪鱼巨噬细胞移动抑制因子（MIF）的克隆与序列分析 |
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| 引用本文： | 毛勇,王军,张之文,王定,苏永全.黄鳍金枪鱼巨噬细胞移动抑制因子（MIF）的克隆与序列分析[J].海洋科学,2009,33(9):29-34. |
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| 作者姓名： | 毛勇 王军 张之文 王定 苏永全 |
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| 作者单位： | 1. 厦门大学,海洋环境学院,福建,厦门,361005;广东海洋大学,水产学院,广东,湛江,524025
2. 厦门大学,海洋环境学院,福建,厦门,361005 |
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| 摘 要： | 采用同源克隆的方法、利用RACE技术从黄鳍金枪鱼(Thunnus albacares)肝脏中克隆了其MIF(TaMIF)的cDNA序列.TaMIF的cDNA全长706 bp含一个345 bp的开放阅读框,编码一个长115氨基酸的蛋白质.信号肽预测分析表明TaMIF没有信号肽.序列分析与进化分析表明,黄鳍金枪鱼与近海养殖鱼类MIF的氨基酸序列高度相似、进化地位相近,预示着深海鱼类TaMIF与近海养殖鱼类具有相似的生物学功能.研究结果是对深海鱼类MIF基因信息资料的重要补充.
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| 关 键 词： | 黄鳍金枪鱼(Thunnus albacares) 巨噬细胞移动抑制因子(MIF) 分子克隆 序列分析 |
| 收稿时间： | 2009/6/12 0:00:00 |
| 修稿时间： | 7/2/2009 12:00:00 AM |
Molecular cloning and sequence analysis of macrophage migration inhibitory factor(MIF) gene from Thunnus albacares |
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| MAO Yong,WANG Jun,ZHANG Zhi-wen,WANG Ding and SU Yong-quan.Molecular cloning and sequence analysis of macrophage migration inhibitory factor(MIF) gene from Thunnus albacares[J].Marine Sciences,2009,33(9):29-34. |
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| Authors: | MAO Yong WANG Jun ZHANG Zhi-wen WANG Ding and SU Yong-quan |
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| Abstract: | The cDNA sequence of an MIF homologue (TaMIF) was cloned from the liver of Thunnus albacares by a homologous cloning strategy and RACE approach. The full length cDNA of TaMIF was 706 bases long and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. Predication of the signal peptide indicated that TaMIF lacks a signal peptide. Sequence analysis revealed a high structural similarity and phylogenetic analysis indicated a close relationship between TaMIF and other fish MIF, implying that TaMIF maybe have a simliar function to offshore cultured fish MIFs. The result is the complement of the gene information to open sea fish MIF gene. |
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