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渤海沉积物中细菌群落结构和基因丰度的空间分布特征
作者姓名:陈泉睿  樊景凤  苏洁  明红霞  孙志浩  李梦飞  赵小慧  王艳涛  张颖雪  张慧珍  金媛  马笑晚  王斌
作者单位:国家海洋环境监测中心, 大连, 116023, 中国;水产与生命学院, 大连海洋大学, 大连116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国;第四海洋研究所, 自然资源部, 北海, 536000, 中国,国家海洋环境监测中心, 大连, 116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国;水产与生命学院, 大连海洋大学, 大连116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国;水产与生命学院, 大连海洋大学, 大连116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国;水产与生命学院, 大连海洋大学, 大连116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国;水产与生命学院, 大连海洋大学, 大连116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国;水产与生命学院, 大连海洋大学, 大连116023, 中国,国家海洋环境监测中心, 大连, 116023, 中国,第四海洋研究所, 自然资源部, 北海, 536000, 中国,第四海洋研究所, 自然资源部, 北海, 536000, 中国,水产与生命学院, 大连海洋大学, 大连116023, 中国
基金项目:The National Key Basic Research Special Foundation of China under contract No. 2017YFC1404500; the National Natural Science Foundation of China under contract No. 41676115.
摘    要:This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants.

关 键 词:Bohai  Sea  SEDIMENTS  bacterial  community  structure  HIGH-THROUGHPUT  SEQUENCING  16S  rRNA  GENE
收稿时间:2018-09-20
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