| 赤点石斑鱼HSP60基因克隆及弧菌应激前后的组织表达特性分析 |
| |
| 引用本文： | 曲朦,施晓峰,张之文,丁少雄.赤点石斑鱼HSP60基因克隆及弧菌应激前后的组织表达特性分析[J].海洋学报,2011,33(1):111-120. |
| |
| 作者姓名： | 曲朦 施晓峰 张之文 丁少雄 |
| |
| 作者单位： | 厦门大学 海洋与环境学院, 福建 厦门 361005 |
| |
| 基金项目： | 福建省自然科学基金(2060203);福建省新世纪人才资助计划。 |
| |
| 摘 要： | HSP60(heat shock protein 60,热休克蛋白60)作为高度保守的热休克蛋白家族的一员,不仅可以在应激状态下帮助其他蛋白正确折叠并恢复天然构象,还可作为危险信号作用于天然免疫系统.基于HSP60的EST序列设计特异引物,采用RACE技术成功克隆获得赤点石斑鱼HSP60 cDNA全序列及基因组全序列....
|
| 关 键 词： | 赤点石斑鱼 HSP60 克隆 组织表达特性 |
| 收稿时间： | 2010/8/23 0:00:00 |
Cloning of HSP60 gene from Epinephelus akaara and its express characterization before and after vibrionic stressed |
| |
| QU Meng,SHI Xiao-feng,ZHANG Zhi-wen and DING Shao-xiong.Cloning of HSP60 gene from Epinephelus akaara and its express characterization before and after vibrionic stressed[J].Acta Oceanologica Sinica (in Chinese),2011,33(1):111-120. |
| |
| Authors: | QU Meng SHI Xiao-feng ZHANG Zhi-wen and DING Shao-xiong |
| |
| Affiliation: | College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China |
| |
| Abstract: | As a major member of heat shock protein families, HSP60 can not only be served as a molecular chaperone to promote protein disaggregation and proper refolding, but also be regarded as a threatened signal under stress to elicit a potent proinflammatory response in cells of the innate immune system. Based on the EST sequences from cDNA library, the full length cDNA of HSP60 from E.akaara was successfully obtained by RACE technique. The full-length of HSP60 is 2351bp, including 75bp of 5'UTR, 539bp of 3'UTR, and a 1737bp open reading frame encoding a protein of 578 amino acids. The alignment result shows that the nuclear acid sequence and amino acids sequence of HSP60 from E. akaara share high identity with other vertebrate homologs. The phylogenetic tree based on the HSP60 amino acid sequences is congruent with their evolutionary relationships, which indicates that HSP60 may be an appropriate marker for the phylogenetic research among interspecies. The E. akaara HSP60 gene contains 9 introns and 10 exons, the same as other teleosteans. The conserved sequence, gene structure and 3D protein structure show that HSP60 may play an important role in life activities. Real-time PCR was also conducted to detect the express characterization of the E. akaara HSP60 gene. The result shows that HSP60 can be detected in various tissues, and after Vibrio harveyi-challenging, the expression of HSP60 in the liver increases dramatically, most of other tissues are also detected increasing to a great extent, which supports that HSP60 maybe play a role in fish innate immune response. |
| |
| Keywords: | Epinephelus akaara heat shock protein60 cloning express characterization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋学报》浏览原始摘要信息 |
|
点击此处可从《海洋学报》下载全文 |
|
