| 基于环境DNA技术的西沙礁区长棘海星种群丰度研究 |
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| 引用本文： | 闫智聪,邢家杰,蔡文启,张开典,吴钟解,李元超,唐佳,周智.基于环境DNA技术的西沙礁区长棘海星种群丰度研究[J].海洋学报,2023,45(3):76-83. |
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| 作者姓名： | 闫智聪 邢家杰 蔡文启 张开典 吴钟解 李元超 唐佳 周智 |
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| 作者单位： | 1.海南大学 海洋学院，海南 海口 570228 |
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| 基金项目： | 海南省自然科学基金（420CXTD432，2019RC067）；国家自然科学基金（42076145）。 |
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| 摘 要： | 长棘海星(Acanthaster planci)作为珊瑚的天敌之一，因其对珊瑚礁生态系统的灾难性破坏而备受关注。然而，长棘海星在南海珊瑚礁生态系统中的时空分布特征仍不清楚。本研究于2020年9月、2021年4月和2022年1月对西沙群岛礁区表层海水进行取样，借助环境DNA(environmental DNA)和实时荧光定量PCR技术分析了表层海水中长棘海星线粒体细胞色素-c-氧化酶亚基I(COTS-mtCOI)基因片段浓度的时空变化，及其与海水温度、盐度、pH、叶绿素含量和营养盐含量等环境因子的相关性。结果发现，2020-2022年，西沙礁区COTS-mtCOI片段浓度的变化范围为0～4.13×107拷贝数/m3，且永乐环礁附近一直有较高的COTS-mtCOI片段浓度。对于华光礁、晋卿岛、羚羊礁、全富岛和赵述岛而言，2020年9月表层海水中COTS-mtCOI片段的平均浓度显著高于2021年4月和2022年1月(p<0.05)。此外，COTS-mtCOI片段浓度与表层海水的温度显著正相关(p<0.05)。研究结果表明，当前长棘海星...
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| 关 键 词： | 珊瑚礁 长棘海星 环境DNA 海水温度 |
| 收稿时间： | 2022-04-10 |
Study on the population distribution of Acanthaster planci in the reef area of the Xisha Islands based on environmental DNA technology |
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| Affiliation: | 1.College of Marine Sciences, Hainan University, Haikou 570228, China2.Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, China |
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| Abstract: | Acanthaster planci, one of the predators of reef-building corals, has attracted much attention for its catastrophic damage to coral reef ecosystems. However, the spatial and temporal distribution characteristics of A. planci are still unclear in the coral reef ecosystem of the South China Sea. In this study, using environmental DNA and real-time quantitative PCR techniques, we analyzed the concentration variation of the mitochondrial cytochrome-c-oxidase subunits I (COTS-mtCOI) fragment of A. planci in the surface seawater of the Xisha Islands in September 2020, April 2021 and January 2022, and the correlations between the concentration variation with environmental factors such as seawater temperature, salinity, pH, chlorophyll content, nutrients content and other environmental factors. The results showed that COTS-mtCOI fragment concentration in the Xisha Islands varied from 0 copies/m3 to 4.13×107 copies/m3 during 2020?2022, and there were always higher concentrations in the Yongle Atoll. For Huaguang Reef, Jinqing Islands, Lingyang Reef, Quanfu Island and Zhaoshu Island, the average concentration of COTS-mtCOI fragment in September 2020 was significantly (p<0.05) higher than those in April 2021 and January 2022. In addition, COTS-mtCOI fragment concentration was significantly (p<0.05) positively correlated with surface seawater temperature. These results suggest that the population of A. planci is widely distributed in the seawater of Xisha Islands, and higher density of A. planci could appear in Yongle Atoll. Moreover, ocean warming may accelerate the outbreak of A. planci. This study is helpful to understand the population distribution characteristics of A. planci in the coral reef ecosystems of the South China Sea, and can provide a theoretical basis for the early warning and forecast of the A. planci outbreak. |
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