| 海洋绿藻气生硬毛藻(Chaetomorpha aerea)和线形硬毛藻(C. linum)的形态与分子鉴定 |
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| 作者姓名： | 黄冰心 滕林宏 丁兰平 |
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| 作者单位： | 天津市动植物抗性重点实验室, 生命科学学院, 天津师范大学, 天津 300387, 中国;中国科学院海洋研究所, 青岛 266071, 中国;汕头大学, 汕头 515063, 中国,中国科学院海洋研究所, 青岛 266071, 中国;中国科学院研究生院, 北京 100049, 中国,天津市动植物抗性重点实验室, 生命科学学院, 天津师范大学, 天津 300387, 中国;中国科学院海洋研究所, 青岛 266071, 中国;汕头大学, 汕头 515063, 中国 |
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| 基金项目： | The National Key Technology R & D Program of China under contract No.2012BAC07B05; the National Natural Science Foundation of China under contract Nos 31400186, 31270257 and 31093440; the Science and Technology Plan Project of Guangdong Province under contract No.2012A020200007; the Science and Technology Plan Project of Shantou City, China under contract No.2012-171. |
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| 摘 要： | Green macroalgae Chaetomorpha aerea and C. linum are taxonomically confused. In this paper, we tried morphological and molecular analyses to separate these two species. C. aerea and C. linum can be distinguished from morphological characteritics, such as frond dimension, cells size and shape, their mean length/width ratios(LWR), and cell walls constriction. Thalli of C. aerea attenuate basipetally, with diameter 270–500 μm at upper portion, 160–360 μm at middle portion, 100–160 μm at basal portion. For the upper part, the length of cells is less than their diameter. Cell walls usually constrict at the dissepiments, which are pellucid or colorless and give the filament beaded appearance. In contrast, thalli of C. linum often have a constant diameter of 90–300 μm within the same individual, cell walls usually do not constrict and cells are cylindrical or barrel shaped. The LWR is larger than that of C. aerea. Results show that the pairwise distance between two species is 3.6%–3.7% for 18 S r RNA gene and 53.5%–54.3% for ITS region. In phylogeny, they distribute at distant clades, which confirms a genetic divergence at molecular level. In addition, morphological data indicates that filament diameter of C. linum samples is highly variable, ranging from 90 μm to 300 μm. Then these two species can be considered as separate species.
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| 关 键 词： | 气生硬毛藻Chaetomorpha aerea 线形硬毛藻Chaetomorpha linum 分子鉴定 形态比较 18S rRNA ITS |
| 收稿时间： | 2015-01-13 |
| 修稿时间： | 2015-08-07 |
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