| 摘要: |
| 从鄂霍次克海沉积物样品中提取总基因组DNA, 进行核糖体rRNA 基因内转录间隔区(ITS)的扩增, 构建克隆文库并进行测序, 对该环境下真核生物的多样性进行了初步研究。结果发现, 该环境下的真核生物具有较高的多样性, 主要为3 大类, 真核藻类、真菌和微型浮游动物。藻类和微型浮游动物的组成与前人在该海域浮游生物生态学的研究相吻合, 而真菌群落具有较高的多样性, 这为该区域真核生物多样性、所参与的生物地球化学过程及生态学意义的研究奠定了基础。 |
| 关键词: 冷泉沉积物 核糖体rRNA 基因内转录间隔区 真核生物多样性 |
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| 基金项目:中国科学院知识创新工程项目——深海极端环境微生物生命过程研究(KZCX2-YW-JC201), 深海沉积物中甲烷代谢相关微生物群落的元基因组学探索性研究(KSCX-2-YW-G-022, 2007-1); 中国大洋协会项目——深部生物圈微生物活动在沉积物和间隙水中的地球化学记录研究(DYXM-115-02-2-17) |
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| Study of eukaryotic diversity in cold seep sediment of the Okhotsk Sea |
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| Abstract: |
| The eukaryotic diversity in the sediment sampled from the Okhotsk Sea was evaluated using direct isolation of internal transcribed spacer (ITS) rRNA genes by PCR and sequencing of cloned fragments. It is shown from the results that the eukaryotic community exhibited high diversity and all of the sequences were from three groups: algae, fungus and microzooplanktons. The constructions of algal and microzooplanktonic communities were consistent with previous ecological research results in this area. Interestingly, we found that the fungal community showed relatively high diversity, which provided clues for further research. |
| Key words: cold seep sediments internal transcribed spacers eukaryotic diversity |
