| 摘要: |
| 目的：建立新胃乃安片中芒柄花素、毛蕊异黄酮、芒柄花苷及毛蕊异黄酮苷4种异黄酮成分的同时鉴别方法，并对其抗氧化活性进行初步筛选研究。方法：采用薄层色谱-生物自显影技术，以甲苯-乙酸乙酯-甲醇（20∶20∶9）为展开剂对上述4种成分进行分离鉴别，以清除2，2-二苯基-1-苦肼基（DPPH）自由基能力对鉴定成分进行抗氧化活性筛选。结果：薄层色谱特征明显，在同一薄层板上能同时鉴别芒柄花素、毛蕊异黄酮、芒柄花苷及毛蕊异黄酮苷4种成分，其中筛选出毛蕊异黄酮和毛蕊异黄酮苷具有清除DPPH自由基的活性。结论：建立的方法操作简单，重现性好，可为新胃乃安片的质量控制及药效物质研究奠定基础。 |
| 关键词: 新胃乃安片 芒柄花素 毛蕊异黄酮 芒柄花苷 毛蕊异黄酮苷 实验研究 |
| DOI： |
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| Simultaneous identification of four isoflavones in Xinweinaian tablets and preliminary screening for antioxidant activities |
| LIANG Xiao-yin，,YAN Ping，,ZHAN Ruo-ting， |
| (Ministry of Education Key Laboratory of Chinese Medicinal Resource from Lingnan，Research Center of Chinese Herbal Resource Science and Engineering & Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicine，Guangzhou University of Chinese Medicine，Guangzhou 510006，Guangdong，China) |
| Abstract: |
| Objective：To investigate the method for the simultaneous identification of formononetin，calycosin，ononin，and calycosin-7-glucoside in Xinweinaian tablets and perform preliminary screening for antioxidant activities.Methods：With toluene-ethyl acetate-methanol（20∶20∶9) as the developing solvent，thin-layer chromatography-bioautography was performed for the isolation and identification of these four constituents，and their antioxidant activities were screened based on the ability to scavenge free radicals of 2，2-diphenyl-1-picryl-hydrazyl-hydrate（DPPH).Results：There were significant features on thin-layer chromatography，and the simultaneous identification of formononetin，calycosin，ononin，and calycosin-7-glucoside was realized on the same thin-layer plate.Calycosin and calycosin-7-glucoside were found to have the activity to scavenge DPPH free radicals.Conclusion：The method established is simple and has good repeatability，and therefore，it lays a foundation for the quality control of Xinweinaian tablets and studies on effective constituents. |
| Key words: Xinweinaian tablet formononetin calycosin ononin calycosin-7-glucoside experimental study |
