引用本文:	冷晓飞,姜海峰,刘小林,王高学,张艳,高洪涛,常亚青.皱纹盘鲍肠道菌群组成及PCR-DGGE 指纹图谱分析[J].海洋科学,2013,37(5):10-14.

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皱纹盘鲍肠道菌群组成及PCR-DGGE 指纹图谱分析
冷晓飞¹, 姜海峰², 刘小林², 王高学², 张艳², 高洪涛¹, 常亚青³
1.大连太平洋海珍品有限公司;2.西北农林科技大学动物科技学院;3.大连海洋大学农业部海洋水产增养殖学重点实验室

摘要:

对养殖和野生皱纹盘鲍(Haliotis discus hannai Ino )不同个体间肠道菌群数量和种类组成进行了研究, 并采用PCR-DGGE 指纹技术对比分析了二者肠道优势菌群的差异。研究结果表明, 养殖和野生皱纹盘鲍肠道好氧及兼性厌氧菌总数分别为(3.50±0.85)×10⁶个/g, (3.03±1.10)×10⁶个/g; 两组鲍鱼肠道优势菌基本相同, 均为弧菌属(Vibrio), 次优势菌均为玫瑰杆菌属(Roseobacter) 和希瓦氏菌属(Shewanella), 在养殖组中检测到芽孢杆菌属(Bacillus), 表明皱纹盘鲍对肠道细菌具有特异选择性。此外, PCR-DGGE 指纹图谱结果表明, 从养殖和野生皱纹盘鲍肠道样品分别获得14 条和12 条扩增条带,其中人工组中有2 条特异性条带; 二者相似性系数(戴斯系数)为92.31%。

关键词: 皱纹盘鲍(Haliotis discus hannai Ino ) 肠道菌群 PCR-DGGE

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基金项目:辽宁省重点科技攻关项目(2011203003)

Microflora in the digestive tract of Haliotis discus hannai Ino and PCR- DGGE fingerprint analysis

Abstract:

Microflora in the intestinal tract of cultured and wild abalones (Haliotis discus hannai Ino) individuals were studied in this paper. PCR-DGGE fingerprint was explored to analyze the differences between the dominant species in intestinal tract of these two groups. Results showed that the total aerobe and facultative anaerobe in the intestinal tract of cultured and wild abalone reached 3.50±0.85×10⁶ and 3.03±1.10×10⁶ cfu/g, respectively. The dominant species was similar. Vibrio was the dominant species, and Shewanella and Roseobacter were the secondary dominant species, Bacillus was detected in the cultured group only, suggesting that abalones have specific selectivity for microflora in the intestinal tract. Additionally, the result from PCR-DGGE fingerprint showed that 14 and 12 bands were observed from cultured and wild abalones, respectively. There were 2 special bands appeared in cultured group. The similarity between the two groups was 92.31%.

Key words: Haliotis discus hannai Ino intestinal tract microflora PCR-DGGE