Abstract: Based on the data of land use/land cover in Hexi region, Gansu Province, the process of land use transformation and associated contribution to the regional eco-environment in Hexi region were measured and quantified spatial-explicitly, the main results and findings include that: 1) the general quality of regional eco-environment has maintained relative stabilization in the past 15 years. However, the trends of both improvement and degradation in eco-environment were coexist, and displayed their spatial heterogeneity in county-level; 2) the transformation among grassland, woodland and cultivated land acted as the key contributors to the changes in regional eco-environment, while the contribution from the urban land use transformations were insignificant in general; 3) the growth of urban land use was rather slow, indicating that the process of urbanization was generally in active in Hexi region. The urban land use transformation occurred more frequently in Jinchang, Jiayuguan, Zhangye and Wuwei, showing the more active process of urbanization in those region; 4) in county level, the impacts of urban land use transformation on the regional eco-environment exhibited the following regional heterogeneity, i.e., the urban land use transformation improved the eco-environment quality where the quality of eco-environment is inferior originally, and reduced the quality of eco-environment in the area with high quality in eco-environment formerly; 5) among those urban land use transformation, the impact of city and industry land use transformation on eco-environmental quality presented the same trends as that of general urban land use, while the transformation associated with township residential land use exhibited the strong impacts on regional eco-environment both positively and adversely; 6) the growing and shrinking of urban land use both show the two-edged effects (i.e., positive and negative) on regional eco-environment in Hexi Region, the growing of urban land use showed similar trends as the general urban land use in its effects on regional eco-environment, while the shirking of urban land use behaved adversely, i.e., they may continuously undermine the eco-environment where the eco-environmental quality is formerly inferior, and enhance the eco-environment where the eco-environmental quality is high originally.